6k8j

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Current revision (10:41, 27 March 2024) (edit) (undo)
 
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<StructureSection load='6k8j' size='340' side='right'caption='[[6k8j]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
<StructureSection load='6k8j' size='340' side='right'caption='[[6k8j]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6k8j]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K8J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6K8J FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6k8j]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K8J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6K8J FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NLRC4, CARD12, CLAN, CLAN1, IPAF, UNQ6189/PRO20215 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6k8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k8j OCA], [https://pdbe.org/6k8j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6k8j RCSB], [https://www.ebi.ac.uk/pdbsum/6k8j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6k8j ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6k8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k8j OCA], [https://pdbe.org/6k8j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6k8j RCSB], [https://www.ebi.ac.uk/pdbsum/6k8j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6k8j ProSAT]</span></td></tr>
</table>
</table>
== Disease ==
== Disease ==
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[[https://www.uniprot.org/uniprot/NLRC4_HUMAN NLRC4_HUMAN]] The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry.
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[https://www.uniprot.org/uniprot/NLRC4_HUMAN NLRC4_HUMAN] The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry.
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/NLRC4_HUMAN NLRC4_HUMAN]] Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis (PubMed:15107016). The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria (By similarity).[UniProtKB:Q3UP24]<ref>PMID:15107016</ref>
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[https://www.uniprot.org/uniprot/NLRC4_HUMAN NLRC4_HUMAN] Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis (PubMed:15107016). The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria (By similarity).[UniProtKB:Q3UP24]<ref>PMID:15107016</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Nod-like receptor (NLR) proteins activate pyroptotic cell death and IL-1 driven inflammation by assembling and activating the inflammasome complex. Closely related sensor proteins NLRP1 and CARD8 undergo unique auto-proteolysis-dependent activation and are implicated in auto-inflammatory diseases; however, their mechanisms of activation are not understood. Here we report the structural basis of how the activating domains (FIIND(UPA)-CARD) of NLRP1 and CARD8 self-oligomerize to assemble distinct inflammasome complexes. Recombinant FIIND(UPA)-CARD of NLRP1 forms a two-layered filament, with an inner core of oligomerized CARD surrounded by an outer ring of FIIND(UPA). Biochemically, self-assembled NLRP1-CARD filaments are sufficient to drive ASC speck formation in cultured human cells-a process that is greatly enhanced by NLRP1-FIIND(UPA) which forms oligomers in vitro. The cryo-EM structures of NLRP1-CARD and CARD8-CARD filaments, solved here at 3.7 A, uncover unique structural features that enable NLRP1 and CARD8 to discriminate between ASC and pro-caspase-1. In summary, our findings provide structural insight into the mechanisms of activation for human NLRP1 and CARD8 and reveal how highly specific signaling can be achieved by heterotypic CARD interactions within the inflammasome complexes.
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Structural basis for distinct inflammasome complex assembly by human NLRP1 and CARD8.,Gong Q, Robinson K, Xu C, Huynh PT, Chong KHC, Tan EYJ, Zhang J, Boo ZZ, Teo DET, Lay K, Zhang Y, Lim JSY, Goh WI, Wright G, Zhong FL, Reversade B, Wu B Nat Commun. 2021 Jan 8;12(1):188. doi: 10.1038/s41467-020-20319-5. PMID:33420028<ref>PMID:33420028</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6k8j" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Human]]
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[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Boo, Z Z]]
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[[Category: Boo ZZ]]
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[[Category: Gong, Q]]
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[[Category: Gong Q]]
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[[Category: Wu, B]]
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[[Category: Wu B]]
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[[Category: Xu, C]]
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[[Category: Xu C]]
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[[Category: Zhang, J]]
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[[Category: Zhang J]]
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[[Category: Filament]]
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[[Category: Signaling protein]]
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Current revision

Structure of NLRC4 CARD filament

PDB ID 6k8j

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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