6xp8

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Current revision (14:51, 6 March 2024) (edit) (undo)
 
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<StructureSection load='6xp8' size='340' side='right'caption='[[6xp8]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
<StructureSection load='6xp8' size='340' side='right'caption='[[6xp8]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6xp8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_35395 Atcc 35395]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XP8 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6xp8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanosarcina_acetivorans Methanosarcina acetivorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XP8 FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xp8 OCA], [https://pdbe.org/6xp8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xp8 RCSB], [https://www.ebi.ac.uk/pdbsum/6xp8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xp8 ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xp8 OCA], [https://pdbe.org/6xp8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xp8 RCSB], [https://www.ebi.ac.uk/pdbsum/6xp8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xp8 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q8TUA9_METAC Q8TUA9_METAC]
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YcaO enzymes catalyze several post-translational modifications on peptide substrates, including thioamidation, which substitutes an amide oxygen with sulfur. Most predicted thioamide-forming YcaO enzymes are encoded adjacent to TfuA, which when present, is required for thioamidation. While activation of the peptide amide backbone is well established for YcaO enzymes, the function of TfuA has remained enigmatic. Here we characterize the TfuA protein involved in methyl-coenzyme M reductase thioamidation and demonstrate that TfuA catalyzes the hydrolysis of thiocarboxylated ThiS (ThiS-COSH), a proteinaceous sulfur donor, and enhances the affinity of YcaO toward the thioamidation substrate. We also report a crystal structure of a TfuA, which displays a new protein fold. Our structural and mutational analyses of TfuA have uncovered conserved binding interfaces with YcaO and ThiS in addition to revealing a hydrolase-like active site featuring a Ser-Lys catalytic pair.
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Functional elucidation of TfuA in peptide backbone thioamidation.,Liu A, Si Y, Dong SH, Mahanta N, Penkala HN, Nair SK, Mitchell DA Nat Chem Biol. 2021 Mar 11. pii: 10.1038/s41589-021-00771-0. doi:, 10.1038/s41589-021-00771-0. PMID:33707784<ref>PMID:33707784</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6xp8" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Atcc 35395]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Dong, S H]]
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[[Category: Methanosarcina acetivorans]]
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[[Category: Nair, S K]]
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[[Category: Dong S-H]]
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[[Category: Biosynthetic protein]]
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[[Category: Nair SK]]
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[[Category: Tfua]]
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[[Category: Thioamidation]]
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[[Category: Ycao]]
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Current revision

The crystal structure of TfuA involved in peptide backbone thioamidation from Methanosarcina acetivorans

PDB ID 6xp8

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OCA

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