We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

1g39

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 09:32, 28 December 2022

WILD-TYPE HNF-1ALPHA DIMERIZATION DOMAIN

Structural highlights

1g39 is a 4 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

HNF1A_MOUSE Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.^[1] ^[2]

Publication Abstract from PubMed

The N-terminal dimerization domain of the transcriptional activator hepatocyte nuclear factor-1alpha (HNF-1alpha) is essential for DNA binding and association of the transcriptional coactivator, DCoH (dimerization cofactor of HNF-1). To investigate the basis for dimerization of HNF-1 proteins, we determined the 1.2 A resolution X-ray crystal structure of the dimerization domain of HNF-1alpha (HNF-p1). Phasing was facilitated by devising a simple synthesis for Fmoc-selenomethionine and substituting leucine residues with selenomethionine. The HNF-1 dimerization domain forms a unique, four-helix bundle that is preserved with localized conformational shifts in the DCoH complex. In three different crystal forms, HNF-p1 displays subtle shifts in the conformation of the interhelix loop and the crossing angle between the amino- and carboxyl-terminal helices. In all three crystal forms, the HNF-p1 dimers pair through an exposed hydrophobic surface that also forms the binding site for DCoH. Conserved core residues in the dimerization domain of the homologous transcriptional regulator HNF-1beta rationalize the functional heterodimerization of the HNF-1alpha and HNF-1beta proteins. Mutations in HNF-1alpha are associated with maturity-onset diabetes of the young type 3 (MODY3), and the structure of HNF-p1 provides insights into the effects of three MODY3 mutations.

High-resolution structure of the HNF-1alpha dimerization domain.,Rose RB, Endrizzi JA, Cronk JD, Holton J, Alber T Biochemistry. 2000 Dec 12;39(49):15062-70. PMID:11106484^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Servitja JM, Pignatelli M, Maestro MA, Cardalda C, Boj SF, Lozano J, Blanco E, Lafuente A, McCarthy MI, Sumoy L, Guigo R, Ferrer J. Hnf1alpha (MODY3) controls tissue-specific transcriptional programs and exerts opposed effects on cell growth in pancreatic islets and liver. Mol Cell Biol. 2009 Jun;29(11):2945-59. doi: 10.1128/MCB.01389-08. Epub 2009 Mar , 16. PMID:19289501 doi:http://dx.doi.org/10.1128/MCB.01389-08
↑ Rose RB, Bayle JH, Endrizzi JA, Cronk JD, Crabtree GR, Alber T. Structural basis of dimerization, coactivator recognition and MODY3 mutations in HNF-1alpha. Nat Struct Biol. 2000 Sep;7(9):744-8. PMID:10966642 doi:10.1038/78966
↑ Rose RB, Endrizzi JA, Cronk JD, Holton J, Alber T. High-resolution structure of the HNF-1alpha dimerization domain. Biochemistry. 2000 Dec 12;39(49):15062-70. PMID:11106484

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1g39"

@@ Line 3: / Line 3: @@
 <StructureSection load='1g39' size='340' side='right'caption='[[1g39]], [[Resolution|resolution]] 1.22&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1g39]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G39 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G39 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1g39]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G39 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G39 FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1f93|1f93]], [[1g2y|1g2y]], [[1g2z|1g2z]]</div></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g39 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g39 OCA], [https://pdbe.org/1g39 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g39 RCSB], [https://www.ebi.ac.uk/pdbsum/1g39 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g39 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g39 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g39 OCA], [https://pdbe.org/1g39 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g39 RCSB], [https://www.ebi.ac.uk/pdbsum/1g39 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g39 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/HNF1A_MOUSE HNF1A_MOUSE]] Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.<ref>PMID:19289501</ref> <ref>PMID:10966642</ref>
+[https://www.uniprot.org/uniprot/HNF1A_MOUSE HNF1A_MOUSE] Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.<ref>PMID:19289501</ref> <ref>PMID:10966642</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 22: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Alber, T]]
+[[Category: Mus musculus]]
-[[Category: Cronk, J D]]
+[[Category: Alber T]]
-[[Category: Endrizzi, J A]]
+[[Category: Cronk JD]]
-[[Category: Holton, J]]
+[[Category: Endrizzi JA]]
-[[Category: Rose, R B]]
+[[Category: Holton J]]
-[[Category: Dimerization domain]]
+[[Category: Rose RB]]
-[[Category: Four-helix bundle]]
-[[Category: Transcription]]
-[[Category: Transcription factor]]

1g39

From Proteopedia

Revision as of 09:32, 28 December 2022

WILD-TYPE HNF-1ALPHA DIMERIZATION DOMAIN

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox