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1gak

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CRYSTAL STRUCTURE OF GREEN ABALONE SP18

Structural highlights

1gak is a 1 chain structure with sequence from Haliotis fulgens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q25063_HALFU

Publication Abstract from PubMed

Sp18 is an 18 kDa protein that is released from abalone sperm during the acrosome reaction. It coats the acrosomal process where it is thought to mediate fusion between sperm and egg cell membranes. Sp18 is evolutionarily related to lysin, a 16 kDa abalone sperm protein that dissolves the vitelline envelope surrounding the egg. The two proteins were generated by gene duplication followed by rapid divergence by positive selection. Here, we present the crystal structure of green abalone sp18 resolved to 1.86 A. Sp18 is composed of a bundle of five alpha-helices with surface clusters of basic and hydrophobic residues, giving it a large dipole moment and making it extremely amphipathic. The large clusters of hydrophobic surface residues and domains of high positive electrostatic surface charge explain sp18's ability as a potent fusagen of liposomes. The overall fold of sp18 is similar to that of green abalone lysin; however, the surface features of the proteins are quite different, accounting for their different roles in fertilization. This is the first crystal structure of a protein implicated in sperm-egg fusion during animal fertilization.

The crystal structure of a fusagenic sperm protein reveals extreme surface properties.,Kresge N, Vacquier VD, Stout CD Biochemistry. 2001 May 8;40(18):5407-13. PMID:11331004^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kresge N, Vacquier VD, Stout CD. The crystal structure of a fusagenic sperm protein reveals extreme surface properties. Biochemistry. 2001 May 8;40(18):5407-13. PMID:11331004

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1gak"

Categories: Haliotis fulgens | Large Structures | Kresge N | Stout CD | Vacquier VD

@@ Line 6: / Line 6: @@
 </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gak OCA], [https://pdbe.org/1gak PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gak RCSB], [https://www.ebi.ac.uk/pdbsum/1gak PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gak ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q25063_HALFU Q25063_HALFU]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 23: @@
 [[Category: Haliotis fulgens]]
 [[Category: Large Structures]]
-[[Category: Kresge, N]]
+[[Category: Kresge N]]
-[[Category: Stout, C D]]
+[[Category: Stout CD]]
-[[Category: Vacquier, V D]]
+[[Category: Vacquier VD]]
-[[Category: Cell adhesion]]
-[[Category: Helical bundle]]

1gak

From Proteopedia

Current revision

CRYSTAL STRUCTURE OF GREEN ABALONE SP18

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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