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6lhd
From Proteopedia
(Difference between revisions)
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==Crystal structure of p53/BCL-xL fusion complex== | ==Crystal structure of p53/BCL-xL fusion complex== | ||
| - | <StructureSection load='6lhd' size='340' side='right'caption='[[6lhd]]' scene=''> | + | <StructureSection load='6lhd' size='340' side='right'caption='[[6lhd]], [[Resolution|resolution]] 2.50Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LHD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LHD FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6lhd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LHD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LHD FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lhd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lhd OCA], [https://pdbe.org/6lhd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lhd RCSB], [https://www.ebi.ac.uk/pdbsum/6lhd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lhd ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">P53 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lhd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lhd OCA], [https://pdbe.org/6lhd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lhd RCSB], [https://www.ebi.ac.uk/pdbsum/6lhd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lhd ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/B2CL1_HUMAN B2CL1_HUMAN]] Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.<ref>PMID:19917720</ref> <ref>PMID:21840391</ref> Isoform Bcl-X(S) promotes apoptosis.<ref>PMID:19917720</ref> <ref>PMID:21840391</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The tumor suppressor p53 is mutated in approximately half of all human cancers. p53 can induce apoptosis through mitochondrial membrane permeabilization by interacting with and antagonizing the anti-apoptotic proteins BCL-xL and BCL-2. However, the mechanisms by which p53 induces mitochondrial apoptosis remain elusive. Here, we report a 2.5 A crystal structure of human p53/BCL-xL complex. In this structure, two p53 molecules interact as a homodimer, and bind one BCL-xL molecule to form a ternary complex with a 2:1 stoichiometry. Mutations at the p53 dimer interface or p53/BCL-xL interface disrupt p53/BCL-xL interaction and p53-mediated apoptosis. Overall, our current findings of the bona fide structure of p53/BCL-xL complex reveal the molecular basis of the interaction between p53 and BCL-xL, and provide insight into p53-mediated mitochondrial apoptosis. | ||
| + | |||
| + | Structural insight into the molecular mechanism of p53-mediated mitochondrial apoptosis.,Wei H, Qu L, Dai S, Li Y, Wang H, Feng Y, Chen X, Jiang L, Guo M, Li J, Chen Z, Chen L, Zhang Y, Chen Y Nat Commun. 2021 Apr 16;12(1):2280. doi: 10.1038/s41467-021-22655-6. PMID:33863900<ref>PMID:33863900</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6lhd" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[B-cell lymphoma proteins 3D structures|B-cell lymphoma proteins 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Human]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Chen Y]] | + | [[Category: Chen, Y]] |
| - | [[Category: Wei H]] | + | [[Category: Wei, H]] |
| + | [[Category: Complex]] | ||
| + | [[Category: Interaction]] | ||
| + | [[Category: Protein binding]] | ||
Revision as of 09:23, 26 May 2021
Crystal structure of p53/BCL-xL fusion complex
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Categories: Human | Large Structures | Chen, Y | Wei, H | Complex | Interaction | Protein binding
