7e7g
From Proteopedia
(Difference between revisions)
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==2-aminoethylphosphonate:pyruvate aminotransferase (AEPT) native== | ==2-aminoethylphosphonate:pyruvate aminotransferase (AEPT) native== | ||
| - | <StructureSection load='7e7g' size='340' side='right'caption='[[7e7g]]' scene=''> | + | <StructureSection load='7e7g' size='340' side='right'caption='[[7e7g]], [[Resolution|resolution]] 2.35Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E7G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E7G FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7e7g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E7G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E7G FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e7g OCA], [https://pdbe.org/7e7g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e7g RCSB], [https://www.ebi.ac.uk/pdbsum/7e7g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e7g ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e7g OCA], [https://pdbe.org/7e7g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e7g RCSB], [https://www.ebi.ac.uk/pdbsum/7e7g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e7g ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PHNW_PSEAE PHNW_PSEAE] Involved in phosphonate degradation. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | 2-aminoethylphosphonate:pyruvate aminotransferase (AEPT) is a pyridoxal 5'-phosphate (PLP)-dependent enzyme that mediates the first step in the AEP degradation pathway. It catalyzes the transamination of 2-aminoethylphosphonate (AEP) with pyruvate to phosphonoacetaldehyde and l-alanine respectively. Although the enzyme is widely present in microorganisms, there are few reports on the structure and function of AEPT to date. Here we report the crystal structure of AEPT from Pseudomonas aeruginosa PAO1 (PaAEPT) to 2.35 A resolution in the absence of the PLP cofactor. PaAEPT crystallizes in space group P21212 with one monomer per asymmetric unit. Analytical ultracentrifugation analysis shows that PaAEPT forms a stable dimer in solution. Our work provides a valuable starting point for further functional and mechanistic studies of the AEP degradation pathway. | ||
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| + | Structural characterization of a 2-aminoethylphosphonate:pyruvate aminotransferase from Pseudomonas aeruginosa PAO1.,Jia H, Chen Y, Chen Y, Liu R, Zhang Q, Bartlam M Biochem Biophys Res Commun. 2021 Mar 17;552:114-119. doi:, 10.1016/j.bbrc.2021.03.046. PMID:33743347<ref>PMID:33743347</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7e7g" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Pseudomonas aeruginosa PAO1]] | ||
[[Category: Bartlam M]] | [[Category: Bartlam M]] | ||
[[Category: Jia H]] | [[Category: Jia H]] | ||
[[Category: Zhang Q]] | [[Category: Zhang Q]] | ||
Current revision
2-aminoethylphosphonate:pyruvate aminotransferase (AEPT) native
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