This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
7kyw
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==Crystal structure of timothy grass allergen Phl p 12.0101 reveals an unusual profilin dimer== | ==Crystal structure of timothy grass allergen Phl p 12.0101 reveals an unusual profilin dimer== | ||
| - | <StructureSection load='7kyw' size='340' side='right'caption='[[7kyw]]' scene=''> | + | <StructureSection load='7kyw' size='340' side='right'caption='[[7kyw]], [[Resolution|resolution]] 2.30Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KYW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KYW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7kyw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Phleum_pratense Phleum pratense]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KYW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KYW FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kyw OCA], [https://pdbe.org/7kyw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kyw RCSB], [https://www.ebi.ac.uk/pdbsum/7kyw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kyw ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kyw OCA], [https://pdbe.org/7kyw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kyw RCSB], [https://www.ebi.ac.uk/pdbsum/7kyw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kyw ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PROF1_PHLPR PROF1_PHLPR] Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Timothy grass pollen is a source of potent allergens. Among them, Phl p 1 and Phl p 5 are thought to be the most important, as a majority of timothy grass-allergic individuals have IgE antibodies directed against these two allergens. The profilin from timothy grass (Phl p 12) has been registered as a minor allergen, with up to 35% of individuals in populations of grass pollen allergic patients showing IgE binding to Phl p 12. Profilins are primarily minor allergens and are known for a high likelihood of co-sensitization as well as cross-reactivity situations caused by their sequence and structure similarity. The crystal structure of Phl p 12.0101 was determined and it revealed that this allergen may form an unusual dimer not previously observed among any profilins. For example, the Phl p 12 dimer has a completely different geometry and interface when compared with the latex profilin (Hev b 8) dimer that has its crystal structure determined. The structure of Phl p 12.0101 is described in the context of allergenic sensitization and allergy diagnostics. Moreover, the structure of the Phl p 12.0101 dimer is discussed, taking into account the production of recombinant allergens and their storage. | ||
| + | |||
| + | Crystal structure of timothy grass allergen Phl p 12.0101 reveals an unusual profilin dimer.,O'Malley A, Kapingidza AB, Hyduke N, Dolamore C, Kowal K, Chruszcz M Acta Biochim Pol. 2021 Mar 15;68(1):15-22. doi: 10.18388/abp.2020_5587. PMID:33720678<ref>PMID:33720678</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7kyw" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Profilin 3D Structures|Profilin 3D Structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Phleum pratense]] | ||
[[Category: Chruszcz M]] | [[Category: Chruszcz M]] | ||
[[Category: Dolamore C]] | [[Category: Dolamore C]] | ||
Current revision
Crystal structure of timothy grass allergen Phl p 12.0101 reveals an unusual profilin dimer
| |||||||||||
