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6kth

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Crystal structure of Juvenile hormone diol kinase JHDK-L2 from silkworm, Bombyx mori

Structural highlights

6kth is a 1 chain structure with sequence from Bombyx mori. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.22Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Juvenile hormone diol kinase (JHDK) is an important enzyme involved in the juvenile hormone metabolism pathway, which catalyzes the phosphorylation of juvenile hormone diol to form the polar metabolite JH diol phosphate. Here, we reported the first crystal structure of insect JHDK from Bombyx mori, BmJHDK-L2, determined at a resolution of 1.22 A. The structure of BmJHDK-L2 mainly comprises of eight alpha-helical segments linked with loops, forming four helix-loop-helix motifs. In these four helix-loop-helix motifs with only one calcium ion bound in the first motif. Circular dichroism spectra indicated that BmJHDK-L2 has strong thermal stability, which is independent of the divalent cation. The structure of BmJHDK-L2 further allowed us to define an ATP-binding site using computational simulation and binding assays, providing a structural basis for development of inhibitor of JHDK. Moreover, the expression profile of BmJHDK-L2 indicated a predominant role in juvenile hormone metabolism in the Malpighian tubules of silkworm. Collectively, these findings expand our knowledge regarding the structural and biochemical features of insect JHDK proteins.

Structural characterization and functional analysis of juvenile hormone diol kinase from the silkworm, Bombyx mori.,Xu H, Zhang Y, Zhang L, Wang Z, Guo P, Zhao P Int J Biol Macromol. 2021 Jan 15;167:570-577. doi:, 10.1016/j.ijbiomac.2020.11.138. Epub 2020 Nov 26. PMID:33249150^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Xu H, Zhang Y, Zhang L, Wang Z, Guo P, Zhao P. Structural characterization and functional analysis of juvenile hormone diol kinase from the silkworm, Bombyx mori. Int J Biol Macromol. 2021 Jan 15;167:570-577. doi:, 10.1016/j.ijbiomac.2020.11.138. Epub 2020 Nov 26. PMID:33249150 doi:http://dx.doi.org/10.1016/j.ijbiomac.2020.11.138

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6kth"

@@ Line 3: / Line 3: @@
 <StructureSection load='6kth' size='340' side='right'caption='[[6kth]], [[Resolution|resolution]] 1.22&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6kth]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bommo Bommo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KTH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KTH FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6kth]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bombyx_mori Bombyx mori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KTH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KTH FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.22&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">JHDK-L2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7091 BOMMO])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kth FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kth OCA], [https://pdbe.org/6kth PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kth RCSB], [https://www.ebi.ac.uk/pdbsum/6kth PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kth ProSAT]</span></td></tr>
 </table>
@@ Line 21: / Line 21: @@
 __TOC__
 </StructureSection>
-[[Category: Bommo]]
+[[Category: Bombyx mori]]
 [[Category: Large Structures]]
-[[Category: Guo, P C]]
+[[Category: Guo PC]]
-[[Category: Wang, Z]]
+[[Category: Wang Z]]
-[[Category: Xu, H Y]]
+[[Category: Xu HY]]
-[[Category: Zhang, L]]
+[[Category: Zhang L]]
-[[Category: Zhang, Y S]]
+[[Category: Zhang YS]]
-[[Category: Zhao, P]]
+[[Category: Zhao P]]
-[[Category: Catabolism]]
-[[Category: Ef-hand motif]]
-[[Category: Hormone]]
-[[Category: Kinase]]

6kth

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Current revision

Crystal structure of Juvenile hormone diol kinase JHDK-L2 from silkworm, Bombyx mori

Structural highlights

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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