1hue

<StructureSection load='1hue' size='340' side='right'caption='[[1hue]], [[NMR_Ensembles_of_Models | 25 NMR models]]' scene=''>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hue FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hue OCA], [https://pdbe.org/1hue PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hue RCSB], [https://www.ebi.ac.uk/pdbsum/1hue PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hue ProSAT]</span></td></tr>

[[https://www.uniprot.org/uniprot/DBH_GEOSE DBH_GEOSE]] Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions.

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== Publication Abstract from PubMed ==

The histone-like protein HU from Bacillus stearothermophilus is a dimer with a molecular mass of 19.5 kDa that is capable of bending DNA. An X-ray structure has been determined, but no structure could be established for a large part of the supposed DNA-binding beta-arms. Using distance and dihedral constraints derived from triple-resonance NMR data of a 13C/15N doubly-labelled HU protein 49 distance geometry structures were calculated, which were refined by means of restrained Molecular Dynamics. From this set a total of 25 refined structures were selected having low constraint energy and few constraint violations. The ensemble of 25 structures display a root-mea-square co-ordinate deviation of 0.36 A with respect to the average structure, calculated over the backbone heavy atoms of residues 2 to 54 and 75 to 90 (and residues 2' to 54' and 75' to 90' of the second monomer). The structure of the core is very similar to that observed in the X-ray structure, with a pairwise r.m.s.d. of 1.06 A. The structure of the beta-hairpin arm contains a double flip-over at the prolines in the two strands of the beta-arm. Strong 15N-NH heteronuclear nuclear Overhauser effects indicate that the beta-arm and especially the tip is flexible. This explains the disorder observed in the solution and X-ray structures of the beta-arm, in respect of the core of the protein. Overlayed onto itself the beta-arm is better defined, with an r.m.s.d. of 1.0 A calculated over the backbone heavy atoms of residues 54 to 59 and 69 to 74. The tip of the arm adopts a well-defined 4:6 beta-hairpin conformation similar to the iron co-ordinating beta-arms of rubredoxin.

Solution structure of the HU protein from Bacillus stearothermophilus.,Vis H, Mariani M, Vorgias CE, Wilson KS, Kaptein R, Boelens R J Mol Biol. 1995 Dec 8;254(4):692-703. PMID:7500343<ref>PMID:7500343</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 1hue" style="background-color:#fffaf0;"></div>

== References ==

<references/>

[[Category: Boelens, R]]

[[Category: Kaptein, R]]

[[Category: Mariani, M]]

[[Category: Vis, H]]

[[Category: Vorgias, C E]]

[[Category: Wilson, K S]]

[[Category: Dna-binding]]