2kcj

From Proteopedia

(Difference between revisions)

Current revision

solution structure of FAPP1 PH domain

Structural highlights

2kcj is a 1 chain structure with sequence from Homo sapiens. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PKHA3_HUMAN Involved in Golgi to cell surface membrane traffic. Induces membrane tubulation. Binds preferentially to phosphatidylinositol 4-phosphate (PtdIns4P).^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The mechanisms underlying Golgi targeting and vesiculation are unknown, although the responsible phosphatidylinositol 4-phosphate (PtdIns(4)P) ligand and four-phosphate-adaptor protein (FAPP) modules have been defined. The micelle-bound structure of the FAPP1 pleckstrin homology domain reveals how its prominent wedge independently tubulates Golgi membranes by leaflet penetration. Mutations compromising the exposed hydrophobicity of full-length FAPP2 abolish lipid monolayer binding and compression. The trafficking process begins with an electrostatic approach, phosphoinositide sampling and perpendicular penetration. Extensive protein contacts with PtdIns(4)P and neighbouring phospholipids reshape the bilayer and initiate tubulation through a conserved wedge with features shared by diverse protein modules.

Structural basis of wedging the Golgi membrane by FAPP pleckstrin homology domains.,Lenoir M, Coskun U, Grzybek M, Cao X, Buschhorn SB, James J, Simons K, Overduin M EMBO Rep. 2010 Apr;11(4):279-84. doi: 10.1038/embor.2010.28. Epub 2010 Mar 19. PMID:20300118^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Godi A, Di Campli A, Konstantakopoulos A, Di Tullio G, Alessi DR, Kular GS, Daniele T, Marra P, Lucocq JM, De Matteis MA. FAPPs control Golgi-to-cell-surface membrane traffic by binding to ARF and PtdIns(4)P. Nat Cell Biol. 2004 May;6(5):393-404. Epub 2004 Apr 25. PMID:15107860 doi:10.1038/ncb1119
↑ Lenoir M, Coskun U, Grzybek M, Cao X, Buschhorn SB, James J, Simons K, Overduin M. Structural basis of wedging the Golgi membrane by FAPP pleckstrin homology domains. EMBO Rep. 2010 Apr;11(4):279-84. doi: 10.1038/embor.2010.28. Epub 2010 Mar 19. PMID:20300118 doi:http://dx.doi.org/10.1038/embor.2010.28

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2kcj"

@@ Line 1: / Line 1: @@
 ==solution structure of FAPP1 PH domain==
-<StructureSection load='2kcj' size='340' side='right'caption='[[2kcj]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2kcj' size='340' side='right'caption='[[2kcj]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2kcj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KCJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KCJ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2kcj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KCJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KCJ FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PLEKHA3, FAPP1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kcj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kcj OCA], [https://pdbe.org/2kcj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kcj RCSB], [https://www.ebi.ac.uk/pdbsum/2kcj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kcj ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/PKHA3_HUMAN PKHA3_HUMAN]] Involved in Golgi to cell surface membrane traffic. Induces membrane tubulation. Binds preferentially to phosphatidylinositol 4-phosphate (PtdIns4P).<ref>PMID:15107860</ref>
+[https://www.uniprot.org/uniprot/PKHA3_HUMAN PKHA3_HUMAN] Involved in Golgi to cell surface membrane traffic. Induces membrane tubulation. Binds preferentially to phosphatidylinositol 4-phosphate (PtdIns4P).<ref>PMID:15107860</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 32: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Coskun, U]]
+[[Category: Coskun U]]
-[[Category: James, J]]
+[[Category: James J]]
-[[Category: Lenoir, M]]
+[[Category: Lenoir M]]
-[[Category: Overduin, M]]
+[[Category: Overduin M]]
-[[Category: Simons, K]]
+[[Category: Simons K]]
-[[Category: Fapp1]]
-[[Category: Lipid-binding]]
-[[Category: Membrane]]
-[[Category: Membrane protein]]
-[[Category: Ph domain]]

2kcj

From Proteopedia

Current revision

solution structure of FAPP1 PH domain

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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