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| | ==Solution Structure of C-terminal AbrB== | | ==Solution Structure of C-terminal AbrB== |
| - | <StructureSection load='2mjg' size='340' side='right'caption='[[2mjg]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='2mjg' size='340' side='right'caption='[[2mjg]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2mjg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacsu Bacsu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MJG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MJG FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2mjg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MJG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MJG FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">abrB, cpsX, BSU00370 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mjg OCA], [https://pdbe.org/2mjg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mjg RCSB], [https://www.ebi.ac.uk/pdbsum/2mjg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mjg ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mjg OCA], [https://pdbe.org/2mjg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mjg RCSB], [https://www.ebi.ac.uk/pdbsum/2mjg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mjg ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/ABRB_BACSU ABRB_BACSU]] Ambiactive repressor and activator of the transcription of genes expressed during the transition state between vegetative growth and the onset of stationary phase and sporulation. It controls the expression of genes spovG and tycA. AbrB binds to the tycA promoter region at two A- and T-rich sites, it may be the sole repressor of tycA transcription.<ref>PMID:2504584</ref>
| + | [https://www.uniprot.org/uniprot/ABRB_BACSU ABRB_BACSU] Ambiactive repressor and activator of the transcription of genes expressed during the transition state between vegetative growth and the onset of stationary phase and sporulation. It controls the expression of genes spovG and tycA. AbrB binds to the tycA promoter region at two A- and T-rich sites, it may be the sole repressor of tycA transcription.<ref>PMID:2504584</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacsu]] | + | [[Category: Bacillus subtilis subsp. subtilis str. 168]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Cavanagh, J]] | + | [[Category: Cavanagh J]] |
| - | [[Category: Olson, A L]] | + | [[Category: Olson AL]] |
| - | [[Category: Thompson, R J]] | + | [[Category: Thompson RJ]] |
| - | [[Category: Tucker, A T]] | + | [[Category: Tucker AT]] |
| - | [[Category: Abrb]]
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| - | [[Category: C-terminus]]
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| - | [[Category: Transcription]]
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| Structural highlights
Function
ABRB_BACSU Ambiactive repressor and activator of the transcription of genes expressed during the transition state between vegetative growth and the onset of stationary phase and sporulation. It controls the expression of genes spovG and tycA. AbrB binds to the tycA promoter region at two A- and T-rich sites, it may be the sole repressor of tycA transcription.[1]
Publication Abstract from PubMed
The AbrB protein from Bacillus subtilis is a DNA-binding global regulator controlling the onset of a vast array of protective functions under stressful conditions. Such functions include biofilm formation, antibiotic production, competence development, extracellular enzyme production, motility, and sporulation. AbrB orthologs are known in a variety of prokaryotic organisms, most notably in all infectious strains of Clostridia, Listeria, and Bacilli. Despite its central role in bacterial response and defense, its structure has been elusive because of its highly dynamic character. Orienting its N- and C-terminal domains with respect to one another has been especially problematic. Here, we have generated a structure of full-length, tetrameric AbrB using nuclear magnetic resonance, chemical crosslinking, and mass spectrometry. We note that AbrB possesses a strip of positive electrostatic potential encompassing its DNA-binding region and that its C-terminal domain aids in DNA binding.
Structure and DNA-binding traits of the transition state regulator AbrB.,Olson AL, Tucker AT, Bobay BG, Soderblom EJ, Moseley MA, Thompson RJ, Cavanagh J Structure. 2014 Nov 4;22(11):1650-6. doi: 10.1016/j.str.2014.08.018. Epub 2014, Oct 9. PMID:25308864[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Strauch MA, Spiegelman GB, Perego M, Johnson WC, Burbulys D, Hoch JA. The transition state transcription regulator abrB of Bacillus subtilis is a DNA binding protein. EMBO J. 1989 May;8(5):1615-21. PMID:2504584
- ↑ Olson AL, Tucker AT, Bobay BG, Soderblom EJ, Moseley MA, Thompson RJ, Cavanagh J. Structure and DNA-binding traits of the transition state regulator AbrB. Structure. 2014 Nov 4;22(11):1650-6. doi: 10.1016/j.str.2014.08.018. Epub 2014, Oct 9. PMID:25308864 doi:http://dx.doi.org/10.1016/j.str.2014.08.018
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