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1e6b

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{{STRUCTURE_1e6b| PDB=1e6b | SCENE= }}
{{STRUCTURE_1e6b| PDB=1e6b | SCENE= }}
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'''CRYSTAL STRUCTURE OF A ZETA CLASS GLUTATHIONE S-TRANSFERASE FROM ARABIDOPSIS THALIANA'''
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===CRYSTAL STRUCTURE OF A ZETA CLASS GLUTATHIONE S-TRANSFERASE FROM ARABIDOPSIS THALIANA===
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==Overview==
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The cis-trans isomerisation of maleylacetoacetate to fumarylacetoacetate is the penultimate step in the tyrosine/phenylalanine catabolic pathway and has recently been shown to be catalysed by glutathione S-transferase enzymes belonging to the zeta class. Given this primary metabolic role it is unsurprising that zeta class glutathione S-transferases are well conserved over a considerable period of evolution, being found in vertebrates, plants, insects and fungi. The structure of this glutathione S-transferase, cloned from Arabidopsis thaliana, has been solved by single isomorphous replacement with anomalous scattering and refined to a final crystallographic R-factor of 19.6% using data from 25.0 A to 1.65 A. The zeta class enzyme adopts the canonical glutathione S-transferase fold and forms a homodimer with each subunit consisting of 221 residues. In agreement with structures of glutathione S-transferases from the theta and phi classes, a serine residue (Ser17) is present in the active site, at a position that would allow it to stabilise the thiolate anion of glutathione. Site-directed mutagenesis of this residue confirms its importance in catalysis. In addition, the role of a highly conserved cysteine residue (Cys19) present in the active site of the zeta class glutathione S-transferase enzymes is discussed.
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(as it appears on PubMed at http://www.pubmed.gov), where 11352584 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11352584}}
==About this Structure==
==About this Structure==
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[[Category: Thom, R.]]
[[Category: Thom, R.]]
[[Category: Transferase]]
[[Category: Transferase]]
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Revision as of 21:13, 30 June 2008

Image:1e6b.png

Template:STRUCTURE 1e6b

CRYSTAL STRUCTURE OF A ZETA CLASS GLUTATHIONE S-TRANSFERASE FROM ARABIDOPSIS THALIANA

Template:ABSTRACT PUBMED 11352584

About this Structure

1E6B is a Single protein structure of sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA.

Reference

The structure of a zeta class glutathione S-transferase from Arabidopsis thaliana: characterisation of a GST with novel active-site architecture and a putative role in tyrosine catabolism., Thom R, Dixon DP, Edwards R, Cole DJ, Lapthorn AJ, J Mol Biol. 2001 May 18;308(5):949-62. PMID:11352584

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