1e6i

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[[Image:1e6i.gif|left|200px]]
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{{Seed}}
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[[Image:1e6i.png|left|200px]]
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{{STRUCTURE_1e6i| PDB=1e6i | SCENE= }}
{{STRUCTURE_1e6i| PDB=1e6i | SCENE= }}
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'''BROMODOMAIN FROM GCN5 COMPLEXED WITH ACETYLATED H4 PEPTIDE'''
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===BROMODOMAIN FROM GCN5 COMPLEXED WITH ACETYLATED H4 PEPTIDE===
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==Overview==
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The bromodomain is an approximately 110 amino acid module found in histone acetyltransferases and the ATPase component of certain nucleosome remodelling complexes. We report the crystal structure at 1.9 A resolution of the Saccharomyces cerevisiae Gcn5p bromodomain complexed with a peptide corresponding to residues 15-29 of histone H4 acetylated at the zeta-N of lysine 16. We show that this bromodomain preferentially binds to peptides containing an N:-acetyl lysine residue. Only residues 16-19 of the acetylated peptide interact with the bromodomain. The primary interaction is the N:-acetyl lysine binding in a cleft with the specificity provided by the interaction of the amide nitrogen of a conserved asparagine with the oxygen of the acetyl carbonyl group. A network of water-mediated H-bonds with protein main chain carbonyl groups at the base of the cleft contributes to the binding. Additional side chain binding occurs on a shallow depression that is hydrophobic at one end and can accommodate charge interactions at the other. These findings suggest that the Gcn5p bromodomain may discriminate between different acetylated lysine residues depending on the context in which they are displayed.
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The line below this paragraph, {{ABSTRACT_PUBMED_11080160}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11080160 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11080160}}
==About this Structure==
==About this Structure==
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[[Category: Histone binding]]
[[Category: Histone binding]]
[[Category: N-acetyl lysine]]
[[Category: N-acetyl lysine]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:43:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:13:58 2008''

Revision as of 21:14, 30 June 2008

Image:1e6i.png

Template:STRUCTURE 1e6i

BROMODOMAIN FROM GCN5 COMPLEXED WITH ACETYLATED H4 PEPTIDE

Template:ABSTRACT PUBMED 11080160

About this Structure

1E6I is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

The structural basis for the recognition of acetylated histone H4 by the bromodomain of histone acetyltransferase gcn5p., Owen DJ, Ornaghi P, Yang JC, Lowe N, Evans PR, Ballario P, Neuhaus D, Filetici P, Travers AA, EMBO J. 2000 Nov 15;19(22):6141-9. PMID:11080160

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