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| | {{STRUCTURE_1e7r| PDB=1e7r | SCENE= }} | | {{STRUCTURE_1e7r| PDB=1e7r | SCENE= }} |
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| - | '''GDP 4-KETO-6-DEOXY-D-MANNOSE EPIMERASE REDUCTASE Y136E'''
| + | ===GDP 4-KETO-6-DEOXY-D-MANNOSE EPIMERASE REDUCTASE Y136E=== |
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| - | ==Overview==
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| - | GDP-4-keto-6-deoxy-d-mannose epimerase/reductase is a bifunctional enzyme responsible for the last step in the biosynthesis of GDP-l-fucose, the substrate of fucosyl transferases. Several cell-surface antigens, including the leukocyte Lewis system and cell-surface antigens in pathogenic bacteria, depend on the availability of GDP-l-fucose for their expression. Therefore, the enzyme is a potential target for therapy in pathological states depending on selectin-mediated cell-to-cell interactions. Previous crystallographic investigations have shown that GDP-4-keto-6-deoxy-d-mannose epimerase/reductase belongs to the short-chain dehydrogenase/reductase protein homology family. The enzyme active-site region is at the interface of an N-terminal NADPH-binding domain and a C-terminal domain, held to bind the substrate. The design, expression and functional characterization of seven site-specific mutant forms of GDP-4-keto-6-deoxy-d-mannose epimerase/reductase are reported here. In parallel, the crystal structures of the native holoenzyme and of three mutants (Ser107Ala, Tyr136Glu and Lys140Arg) have been investigated and refined at 1. 45-1.60 A resolution, based on synchrotron data (R-factors range between 12.6 % and 13.9 %). The refined protein models show that besides the active-site residues Ser107, Tyr136 and Lys140, whose mutations impair the overall enzymatic activity and may affect the coenzyme binding mode, side-chains capable of proton exchange, located around the expected substrate (GDP-4-keto-6-deoxy-d-mannose) binding pocket, are selectively required during the epimerization and reduction steps. Among these, Cys109 and His179 may play a primary role in proton exchange between the enzyme and the epimerization catalytic intermediates. Finally, the additional role of mutated active-site residues involved in substrate recognition and in enzyme stability has been analyzed.
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Red]] | | [[Category: Red]] |
| | [[Category: Sdr]] | | [[Category: Sdr]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:46:13 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:17:23 2008'' |
Revision as of 21:17, 30 June 2008
Template:STRUCTURE 1e7r
GDP 4-KETO-6-DEOXY-D-MANNOSE EPIMERASE REDUCTASE Y136E
Template:ABSTRACT PUBMED 11021971
About this Structure
1E7R is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Probing the catalytic mechanism of GDP-4-keto-6-deoxy-d-mannose Epimerase/Reductase by kinetic and crystallographic characterization of site-specific mutants., Rosano C, Bisso A, Izzo G, Tonetti M, Sturla L, De Flora A, Bolognesi M, J Mol Biol. 2000 Oct 13;303(1):77-91. PMID:11021971
Page seeded by OCA on Tue Jul 1 00:17:23 2008
