1m1f
From Proteopedia
(Difference between revisions)
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<StructureSection load='1m1f' size='340' side='right'caption='[[1m1f]], [[Resolution|resolution]] 1.40Å' scene=''> | <StructureSection load='1m1f' size='340' side='right'caption='[[1m1f]], [[Resolution|resolution]] 1.40Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[1m1f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[1m1f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M1F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M1F FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m1f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m1f OCA], [https://pdbe.org/1m1f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m1f RCSB], [https://www.ebi.ac.uk/pdbsum/1m1f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m1f ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m1f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m1f OCA], [https://pdbe.org/1m1f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m1f RCSB], [https://www.ebi.ac.uk/pdbsum/1m1f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m1f ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | + | [https://www.uniprot.org/uniprot/PEMK_ECOLX PEMK_ECOLX] Toxic component of a type II toxin-antitoxin (TA) module. Probably functions as an endoribonuclease. Responsible for the stable maintenance of the plasmid during cell division by postsegregational killing of plasmid-less daughter cells. Neutralized by coexpression with cognate antitoxin PemI. Both PemI and PemK proteins bind to the promoter region of the pem operon to autoregulate their synthesis.<ref>PMID:2832364</ref> <ref>PMID:8455570</ref> | |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m1f ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m1f ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | We have determined the structure of Kid toxin protein from E. coli plasmid R1 involved in stable plasmid inheritance by postsegregational killing of plasmid-less daughter cells. Kid forms a two-component system with its antagonist, Kis antitoxin. Our 1.4 A crystal structure of Kid reveals a 2-fold symmetric dimer that closely resembles the DNA gyrase-inhibitory toxin protein CcdB from E. coli F plasmid despite the lack of any notable sequence similarity. Analysis of nontoxic mutants of Kid suggests a target interaction interface associated with toxicity that is in marked contrast to that proposed for CcdB. A possible region for interaction of Kid with the antitoxin is proposed that overlaps with the target binding site and may explain the mode of antitoxin action. | ||
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| - | Structural and functional analysis of the kid toxin protein from E. coli plasmid R1.,Hargreaves D, Santos-Sierra S, Giraldo R, Sabariegos-Jareno R, de la Cueva-Mendez G, Boelens R, Diaz-Orejas R, Rafferty JB Structure. 2002 Oct;10(10):1425-33. PMID:12377128<ref>PMID:12377128</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 1m1f" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Escherichia coli]] |
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Boelens | + | [[Category: Boelens R]] |
| - | + | [[Category: Diaz-Orejas R]] | |
| - | [[Category: Diaz-Orejas | + | [[Category: Giraldo R]] |
| - | [[Category: Giraldo | + | [[Category: Hargreaves D]] |
| - | [[Category: Hargreaves | + | [[Category: Rafferty JB]] |
| - | [[Category: Rafferty | + | [[Category: Sabariegos-Jareno R]] |
| - | [[Category: Sabariegos-Jareno | + | [[Category: Santos-Sierra S]] |
| - | [[Category: Santos-Sierra | + | [[Category: De la Cueva-Mendez G]] |
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Current revision
Kid toxin protein from E.coli plasmid R1
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