1e8r

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[[Image:1e8r.gif|left|200px]]
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{{Seed}}
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[[Image:1e8r.png|left|200px]]
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{{STRUCTURE_1e8r| PDB=1e8r | SCENE= }}
{{STRUCTURE_1e8r| PDB=1e8r | SCENE= }}
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'''SOLUTION STRUCTURE OF TYPE X CBD'''
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===SOLUTION STRUCTURE OF TYPE X CBD===
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==Overview==
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Plant cell wall hydrolases generally have a modular structure consisting of a catalytic domain linked to one or more noncatalytic carbohydrate-binding modules (CBMs), whose common function is to attach the enzyme to the polymeric substrate. Xylanase A from Pseudomonas fluorescens subsp. cellulosa (Pf Xyn10A) consists of a family 10 catalytic domain, an N-terminal family IIa cellulose-binding module, and an internal family 10 cellulose-binding module. The structure of the 45-residue family 10 CBM has been determined in solution using NMR. It consists of two antiparallel beta-sheets, one with two strands and one with three, with a short alpha-helix across one face of the three-stranded sheet. There is a high density of aromatic residues on one side of the protein, including three aromatic residues (Tyr8, Trp22, and Trp24), which are exposed and form a flat surface on one face, in a classical polysaccharide-binding arrangement. The fold is closely similar to that of the oligonucleotide/oligosaccharide-binding (OB) fold, but appears to have arisen by convergent evolution, because there is no sequence similarity, and the presumed binding sites are on different faces.
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The line below this paragraph, {{ABSTRACT_PUBMED_10653641}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 10653641 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10653641}}
==About this Structure==
==About this Structure==
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1E8R is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ct7 1ct7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E8R OCA].
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1E8R is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ct7 1ct7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E8R OCA].
==Reference==
==Reference==
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[[Category: Beta strand]]
[[Category: Beta strand]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:48:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:19:51 2008''

Revision as of 21:19, 30 June 2008

Image:1e8r.png

Template:STRUCTURE 1e8r

SOLUTION STRUCTURE OF TYPE X CBD

Template:ABSTRACT PUBMED 10653641

About this Structure

1E8R is a Single protein structure of sequence from Pseudomonas fluorescens. This structure supersedes the now removed PDB entry 1ct7. Full experimental information is available from OCA.

Reference

Solution structure of the CBM10 cellulose binding module from Pseudomonas xylanase A., Raghothama S, Simpson PJ, Szabo L, Nagy T, Gilbert HJ, Williamson MP, Biochemistry. 2000 Feb 8;39(5):978-84. PMID:10653641

Page seeded by OCA on Tue Jul 1 00:19:51 2008

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