7bzh
From Proteopedia
(Difference between revisions)
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==Solution structure of a DNA binding protein from Sulfolobus islandicus== | ==Solution structure of a DNA binding protein from Sulfolobus islandicus== | ||
| - | <StructureSection load='7bzh' size='340' side='right'caption='[[7bzh]]' scene=''> | + | <StructureSection load='7bzh' size='340' side='right'caption='[[7bzh]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full | + | <table><tr><td colspan='2'>[[7bzh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"sulfolobus_islandicus"_zillig_et_al._1994 "sulfolobus islandicus" zillig et al. 1994]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BZH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BZH FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bzh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bzh OCA], [https://pdbe.org/7bzh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bzh RCSB], [https://www.ebi.ac.uk/pdbsum/7bzh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bzh ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bzh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bzh OCA], [https://pdbe.org/7bzh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bzh RCSB], [https://www.ebi.ac.uk/pdbsum/7bzh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bzh ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The winged helix superfamily comprises a large number of structurally related nucleic acid-binding proteins. While these proteins are often shown to bind dsDNA, few are known to bind ssDNA. Here, we report the identification and characterization of Sul7s, a novel winged-helix single-stranded DNA binding protein family highly conserved in Sulfolobaceae. Sul7s from Sulfolobus islandicus binds ssDNA with an affinity approximately 15-fold higher than that for dsDNA in vitro. It prefers binding oligo(dT)30 over oligo(dC)30 or a dG-rich 30-nt oligonucleotide, and barely binds oligo(dA)30. Further, binding by Sul7s inhibits DNA strand annealing, but shows little effect on the melting temperature of DNA duplexes. The solution structure of Sul7s determined by NMR shows a winged helix-turn-helix fold, consisting of three alpha-helices, three beta-strands, and two short wings. It interacts with ssDNA via a large positively charged binding surface, presumably resulting in ssDNA deformation. Our results shed significant light on not only non-OB fold single-stranded DNA binding proteins in Archaea, but also the divergence of the winged-helix proteins in both function and structure during evolution. | ||
| + | |||
| + | A Novel Family of Winged-Helix Single-Stranded DNA-Binding Proteins from Archaea.,Huang C, Liu X, Chen Y, Zhou J, Li W, Ding N, Huang L, Chen J, Zhang Z Int J Mol Sci. 2022 Mar 22;23(7). pii: ijms23073455. doi: 10.3390/ijms23073455. PMID:35408816<ref>PMID:35408816</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7bzh" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Sulfolobus islandicus zillig et al. 1994]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Liu | + | [[Category: Liu, X]] |
| - | [[Category: Zhang | + | [[Category: Zhang, Z]] |
| + | [[Category: Archaea]] | ||
| + | [[Category: Crenarchaea]] | ||
| + | [[Category: Dna binding protein]] | ||
| + | [[Category: Sulfolobus]] | ||
Revision as of 10:20, 18 May 2022
Solution structure of a DNA binding protein from Sulfolobus islandicus
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