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7ear
From Proteopedia
(Difference between revisions)
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==A positively charged mutant Cry3Aa endotoxin== | ==A positively charged mutant Cry3Aa endotoxin== | ||
| - | <StructureSection load='7ear' size='340' side='right'caption='[[7ear]]' scene=''> | + | <StructureSection load='7ear' size='340' side='right'caption='[[7ear]], [[Resolution|resolution]] 2.20Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EAR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EAR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7ear]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thuringiensis Bacillus thuringiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EAR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EAR FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ear FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ear OCA], [https://pdbe.org/7ear PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ear RCSB], [https://www.ebi.ac.uk/pdbsum/7ear PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ear ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ear FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ear OCA], [https://pdbe.org/7ear PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ear RCSB], [https://www.ebi.ac.uk/pdbsum/7ear PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ear ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q9S6N9_BACTU Q9S6N9_BACTU] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Direct delivery of proteins into cells holds significant potential for basic research and drug development. However, the poor endosomal escape of conventional delivery strategies remains a challenge, thus limiting the clinical translation of many protein therapeutics. Herein, we report that engineered Cry3Aa protein (Pos3Aa) crystals formed naturally within Bacillus thuringiensis can serve as a vehicle for efficient cytosolic delivery of bioactive proteins. We showed that Pos3Aa-mediated delivery of tumor suppressor p53 protein, a promising therapeutic candidate found to be inactivated in nearly half of human cancers, resulted in the restoration of p53 function in p53-deficient cancer cells, and thereby sensitized them to 5-fluorouracil chemotherapy as demonstrated in in vitro and in vivo models. Our results validate that Pos3Aa crystals can be a robust and effective platform for the cytosolic delivery of effector proteins, and suggest that efficient uptake and endosomal escape could be critical for efficacious p53 protein-based cancer therapy. | ||
| + | |||
| + | Efficient intracellular delivery of p53 protein by engineered protein crystals restores tumor suppressing function in vivo.,Yang Z, Lee MMM, Chan MK Biomaterials. 2021 Apr;271:120759. doi: 10.1016/j.biomaterials.2021.120759. Epub , 2021 Mar 16. PMID:33798968<ref>PMID:33798968</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7ear" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Pesticidal crystal protein|Pesticidal crystal protein]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Bacillus thuringiensis]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Chan MK]] | [[Category: Chan MK]] | ||
[[Category: Lee MM]] | [[Category: Lee MM]] | ||
[[Category: Yang Z]] | [[Category: Yang Z]] | ||
Current revision
A positively charged mutant Cry3Aa endotoxin
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