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7lt8
From Proteopedia
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==Crystal structure of Ras suppressor-1== | ==Crystal structure of Ras suppressor-1== | ||
| - | <StructureSection load='7lt8' size='340' side='right'caption='[[7lt8]]' scene=''> | + | <StructureSection load='7lt8' size='340' side='right'caption='[[7lt8]], [[Resolution|resolution]] 1.76Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LT8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LT8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7lt8]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LT8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LT8 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lt8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lt8 OCA], [https://pdbe.org/7lt8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lt8 RCSB], [https://www.ebi.ac.uk/pdbsum/7lt8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lt8 ProSAT]</span></td></tr> | + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7lt9|7lt9]]</div></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lt8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lt8 OCA], [https://pdbe.org/7lt8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lt8 RCSB], [https://www.ebi.ac.uk/pdbsum/7lt8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lt8 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/RSU1_HUMAN RSU1_HUMAN]] Potentially plays a role in the Ras signal transduction pathway. Capable of suppressing v-Ras transformation in vitro. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Ras suppressor-1 (Rsu-1) is a leucine-rich repeat (LRR)-containing protein that is crucial for regulating cell adhesion, and is involved in such physiological and pathological processes as focal adhesion assembly and tumor metastasis. Rsu-1 interacts with zinc-finger type multi-LIM domain-containing adaptor protein PINCH-1, known to be involved in the integrin-mediated consensus adhesome, but not with its highly homologous family member PINCH-2. However, the structural basis for and regulatory mechanisms of this specific interaction remain unclear. Here, we determined the crystal structures of Rsu-1 and its complex with the PINCH-1 LIM4-5 domains. Rsu-1 displays an arc-shaped solenoid architecture, with eight LRRs shielded by N- and C-terminal capping modules. We showed that the conserved concave surface of the Rsu-1 LRR domain binds and stabilizes the PINCH-1 LIM5 domain via salt bridge and hydrophobic interactions, while the C-terminal non-LIM region of PINCH-2 sterically disfavors Rsu-1 binding. We also showed that Rsu-1 can be assembled, via PINCH-1-binding, into a hetero-pentamer complex comprising Rsu-1, PINCH-1, ILK, Parvin, and Kindlin-2, which constitute a major consensus integrin adhesome crucial for focal adhesion assembly. Our mutagenesis and cell biological data emphasize the significance of the Rsu-1/PINCH-1 interaction in focal adhesion assembly and cell spreading, providing crucial molecular insights into Rsu-1-mediated cell adhesion with implications for disease development. | ||
| + | |||
| + | Molecular basis for Ras suppressor-1 binding to PINCH-1 in focal adhesion assembly.,Fukuda K, Lu F, Qin J J Biol Chem. 2021 Apr 20:100685. doi: 10.1016/j.jbc.2021.100685. PMID:33891945<ref>PMID:33891945</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7lt8" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Fukuda K]] | + | [[Category: Fukuda, K]] |
| - | [[Category: Qin J]] | + | [[Category: Qin, J]] |
| + | [[Category: Cell adhesion]] | ||
| + | [[Category: Leucine-rich repeat]] | ||
Revision as of 09:15, 5 May 2021
Crystal structure of Ras suppressor-1
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