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1hg7

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High resolution structure of HPLC-12 type III antifreeze protein from Ocean Pout Macrozoarces americanus

Structural highlights

1hg7 is a 1 chain structure with sequence from Zoarces americanus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.15Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ANP12_ZOAAM Contributes to protect fish blood from freezing at subzero sea water temperatures. Lowers the blood freezing point. Binds to nascent ice crystals and prevents further growth.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Type III antifreeze proteins (AFPs) are present in the body fluids of some polar fishes where they inhibit ice growth at subzero temperatures. Previous studies of the structure of type III AFP by NMR and X-ray identified a remarkably flat surface on the protein containing amino acids that were demonstrated to be important for interaction with ice by mutational studies. It was proposed that this protein surface binds onto the (1 0 [\bar 1] 0) plane of ice with the key amino acids interacting directly with the water molecules in the ice crystal. Here, we show that the mechanism of type III AFP interaction with ice crystals is more complex than that proposed previously. We report a high-resolution X-ray structure of type III AFP refined at 1.15 A resolution with individual anisotropic temperature factors. We report the results of ice-etching experiments that show a broad surface coverage, suggesting that type III AFP binds to a set of planes that are parallel with or inclined at a small angle to the crystallographic c-axis of the ice crystal. Our modelling studies, performed with the refined structure, confirm that type III AFP can make energetically favourable interactions with several ice surfaces.

Understanding the mechanism of ice binding by type III antifreeze proteins.,Antson AA, Smith DJ, Roper DI, Lewis S, Caves LS, Verma CS, Buckley SL, Lillford PJ, Hubbard RE J Mol Biol. 2001 Jan 26;305(4):875-89. PMID:11162099^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Antson AA, Smith DJ, Roper DI, Lewis S, Caves LS, Verma CS, Buckley SL, Lillford PJ, Hubbard RE. Understanding the mechanism of ice binding by type III antifreeze proteins. J Mol Biol. 2001 Jan 26;305(4):875-89. PMID:11162099 doi:10.1006/jmbi.2000.4336

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1hg7"

@@ Line 3: / Line 3: @@
 <StructureSection load='1hg7' size='340' side='right'caption='[[1hg7]], [[Resolution|resolution]] 1.15&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1hg7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Blennius_americanus Blennius americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HG7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HG7 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1hg7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Zoarces_americanus Zoarces americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HG7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HG7 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.15&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ame|1ame]], [[1b7i|1b7i]], [[1b7j|1b7j]], [[1b7k|1b7k]], [[1ekl|1ekl]], [[1gzi|1gzi]], [[1jab|1jab]], [[1kde|1kde]], [[1kdf|1kdf]], [[1msi|1msi]], [[1msj|1msj]], [[2ame|2ame]], [[2jia|2jia]], [[2msi|2msi]], [[2msj|2msj]], [[2spg|2spg]], [[3ame|3ame]], [[3msi|3msi]], [[4ame|4ame]], [[4msi|4msi]], [[5msi|5msi]], [[6ame|6ame]], [[6msi|6msi]], [[7ame|7ame]], [[7msi|7msi]], [[8ame|8ame]], [[8msi|8msi]], [[9ame|9ame]], [[9msi|9msi]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RECOMBINANT TYPE III AFP HPURCE 10 FRACTION 12 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8199 Blennius americanus])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hg7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hg7 OCA], [https://pdbe.org/1hg7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hg7 RCSB], [https://www.ebi.ac.uk/pdbsum/1hg7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hg7 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/ANP12_ZOAAM ANP12_ZOAAM] Contributes to protect fish blood from freezing at subzero sea water temperatures. Lowers the blood freezing point. Binds to nascent ice crystals and prevents further growth.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 35: / Line 36: @@
 __TOC__
 </StructureSection>
-[[Category: Blennius americanus]]
 [[Category: Large Structures]]
-[[Category: Antson, A A]]
+[[Category: Zoarces americanus]]
-[[Category: Buckley, S L]]
+[[Category: Antson AA]]
-[[Category: Caves, L S.D]]
+[[Category: Buckley SL]]
-[[Category: Hubbard, R E]]
+[[Category: Caves LSD]]
-[[Category: Lewis, S]]
+[[Category: Hubbard RE]]
-[[Category: Lillford, P J]]
+[[Category: Lewis S]]
-[[Category: Roper, D I]]
+[[Category: Lillford PJ]]
-[[Category: Smith, D J]]
+[[Category: Roper DI]]
-[[Category: Verma, C S]]
+[[Category: Smith DJ]]
-[[Category: Antifreeze protein]]
+[[Category: Verma CS]]
-[[Category: Ice-binding protein]]
-[[Category: Macrozoarces americanus]]
-[[Category: Ocean pout]]

1hg7

From Proteopedia

Current revision

High resolution structure of HPLC-12 type III antifreeze protein from Ocean Pout Macrozoarces americanus

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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