This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1oik
From Proteopedia
(Difference between revisions)
| Line 4: | Line 4: | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1oik]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OIK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OIK FirstGlance]. <br> | <table><tr><td colspan='2'>[[1oik]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OIK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OIK FirstGlance]. <br> | ||
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=C26:(2R)-2-ETHYL-1-HEXANESULFONIC+ACID'>C26</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.06Å</td></tr> |
| - | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=C26:(2R)-2-ETHYL-1-HEXANESULFONIC+ACID'>C26</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr> | |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oik FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oik OCA], [https://pdbe.org/1oik PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oik RCSB], [https://www.ebi.ac.uk/pdbsum/1oik PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oik ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oik FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oik OCA], [https://pdbe.org/1oik PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oik RCSB], [https://www.ebi.ac.uk/pdbsum/1oik PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oik ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/ATSK_PSEPU ATSK_PSEPU] Catalyzes the oxigenolytic cleavage of 2-ethylhexyl sulfate (2-EHS) in the presence of alpha-ketoglutarate to yield 2-ethyl-hexanal and succinate, the decarboxylated form of alpha-ketoglutarate. It can accepte a wide range of alpha-keto acids including 2-oxo-valerate, 2-oxo-adipate, 2-oxo-octanoate, 3-methyl-2-oxo-butyrate, oxaloacetate-alpha-ketoadipate, and alpha-ketooctanoate. It can catalyze the cleavage of medium-chain alkyl sulfate esters such as butylsulfate, pentylsulfate, hexylsulfate, heptylsulfate, octylsulfate, nonylsulfate, decylsulfate and sodium dodecyl sulfate (SDS).<ref>PMID:10913158</ref> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
| Line 36: | Line 38: | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Pseudomonas putida]] | [[Category: Pseudomonas putida]] | ||
| - | [[Category: Dierks | + | [[Category: Dierks T]] |
| - | [[Category: Kahnert | + | [[Category: Kahnert A]] |
| - | [[Category: Kertesz | + | [[Category: Kertesz MA]] |
| - | [[Category: Meyer-Klauke | + | [[Category: Meyer-Klauke W]] |
| - | [[Category: Mueller | + | [[Category: Mueller I]] |
| - | [[Category: Pape | + | [[Category: Pape T]] |
| - | [[Category: Uson | + | [[Category: Uson I]] |
| - | + | ||
| - | + | ||
Current revision
Crystal structure of the alkylsulfatase AtsK, a non-heme Fe(II) alphaketoglutarate dependent Dioxygenase in complex with fe, alphaketoglutarate and 2-ethyl-1-hexanesulfuric acid
| |||||||||||
