6zip
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==Crystal Structure of Two-Domain Laccase mutant R240A from Streptomyces griseoflavus== | ==Crystal Structure of Two-Domain Laccase mutant R240A from Streptomyces griseoflavus== | ||
| - | <StructureSection load='6zip' size='340' side='right'caption='[[6zip]]' scene=''> | + | <StructureSection load='6zip' size='340' side='right'caption='[[6zip]], [[Resolution|resolution]] 2.05Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZIP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZIP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6zip]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_griseoflavus Streptomyces griseoflavus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZIP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZIP FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zip OCA], [https://pdbe.org/6zip PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zip RCSB], [https://www.ebi.ac.uk/pdbsum/6zip PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zip ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=PER:PEROXIDE+ION'>PER</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zip OCA], [https://pdbe.org/6zip PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zip RCSB], [https://www.ebi.ac.uk/pdbsum/6zip PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zip ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A0M4FJ81_9ACTN A0A0M4FJ81_9ACTN] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Multi-copper oxidases are capable of coupling the one-electron oxidation of four substrate equivalents to the four-electron reduction of dioxygen to two molecules of water. This process takes place at the trinuclear copper center of the enzymes. Previously, the main catalytic stages for three-domain (3D) laccases have been identified. However, for bacterial small two-domain (2D) laccases several questions remain to be answered. One of them is the nature of the protonation events upon the reductive cleavage of dioxygen to water. In 3D laccases, acidic residues play a key role in the protonation mechanisms. In this study, the role of the Arg240 residue, located within the T2 tunnel of 2D laccase from Streptomyces griseoflavus Ac-993, was investigated. X-ray structural analysis and kinetic characterization of two mutants, R240A and R240H, have provided support for a role of this residue in the protonation events. Communicated by Ramaswamy H. Sarma. | ||
| + | |||
| + | The role of positive charged residue in the proton-transfer mechanism of two-domain laccase from Streptomyces griseoflavus Ac-993.,Gabdulkhakov A, Kolyadenko I, Oliveira P, Tamagnini P, Mikhaylina A, Tishchenko S J Biomol Struct Dyn. 2021 Apr 19:1-8. doi: 10.1080/07391102.2021.1911852. PMID:33870857<ref>PMID:33870857</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6zip" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Laccase 3D structures|Laccase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Streptomyces griseoflavus]] | ||
[[Category: Gabdulkhakov AG]] | [[Category: Gabdulkhakov AG]] | ||
[[Category: Kolyadenko IA]] | [[Category: Kolyadenko IA]] | ||
[[Category: Tishchenko TV]] | [[Category: Tishchenko TV]] | ||
Revision as of 10:05, 21 December 2022
Crystal Structure of Two-Domain Laccase mutant R240A from Streptomyces griseoflavus
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