7ckl

==Structure of Lassa virus polymerase bound to Z matrix protein==

<StructureSection load='7ckl' size='340' side='right'caption='[[7ckl]], [[Resolution|resolution]] 3.88&Aring;' scene=''>

<table><tr><td colspan='2'>[[7ckl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mammarenavirus_lassaense Mammarenavirus lassaense]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CKL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CKL FirstGlance]. <br>

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.88&#8491;</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ckl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ckl OCA], [https://pdbe.org/7ckl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ckl RCSB], [https://www.ebi.ac.uk/pdbsum/7ckl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ckl ProSAT]</span></td></tr>

== Function ==

[https://www.uniprot.org/uniprot/A0A097F4L1_LASV A0A097F4L1_LASV] RNA-dependent RNA polymerase, which is responsible for the replication and transcription of the viral RNA genome using antigenomic RNA as an intermediate. During transcription, synthesizes subgenomic RNAs and assures their capping by a cap-snatching mechanism, which involves the endonuclease activity cleaving the host capped pre-mRNAs. These short capped RNAs are then used as primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner.[HAMAP-Rule:MF_04086][PIRNR:PIRNR000836]

==See Also==

*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]

[[Category: Mammarenavirus lassaense]]

[[Category: Xu X]]