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| | ==The NMR solution structure of the peptide AC12 from Hypsiboas raniceps== | | ==The NMR solution structure of the peptide AC12 from Hypsiboas raniceps== |
| - | <StructureSection load='6fgm' size='340' side='right'caption='[[6fgm]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='6fgm' size='340' side='right'caption='[[6fgm]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6fgm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hypsiboas_raniceps Hypsiboas raniceps]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FGM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6FGM FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6fgm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Boana_raniceps Boana raniceps]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FGM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6FGM FirstGlance]. <br> |
| | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6fgm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fgm OCA], [https://pdbe.org/6fgm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6fgm RCSB], [https://www.ebi.ac.uk/pdbsum/6fgm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6fgm ProSAT]</span></td></tr> | | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6fgm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fgm OCA], [https://pdbe.org/6fgm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6fgm RCSB], [https://www.ebi.ac.uk/pdbsum/6fgm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6fgm ProSAT]</span></td></tr> |
| | </table> | | </table> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Hypsiboas raniceps]] | + | [[Category: Boana raniceps]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Andrade, P B]] | + | [[Category: Andrade PB]] |
| - | [[Category: Bocca, A L]] | + | [[Category: Bocca AL]] |
| - | [[Category: Goodfellow, B J]] | + | [[Category: Goodfellow BJ]] |
| - | [[Category: Jr, P H.H Veloso]]
| + | [[Category: Pereira D]] |
| - | [[Category: Pereira, D]] | + | [[Category: Pereira PJB]] |
| - | [[Category: Pereira, P J.B]] | + | [[Category: Popov CSFC]] |
| - | [[Category: Popov, C S.F C]] | + | [[Category: Rezende TMB]] |
| - | [[Category: Rezende, T M.B]] | + | [[Category: Rodrigues JE]] |
| - | [[Category: Rodrigues, J E]] | + | [[Category: Simas BS]] |
| - | [[Category: Simas, B S]] | + | [[Category: Valentao P]] |
| - | [[Category: Valentao, P]] | + | [[Category: Veloso Jr PHH]] |
| - | [[Category: Ac12 peptide]] | + | |
| - | [[Category: Immune system]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Inflammation is a natural defense mechanism of the immune system; however, when unregulated, it can lead to chronic illness. Glucocorticoids are the most commonly used agents to effectively treat inflammatory conditions, including autoimmune diseases, however these substances can trigger a number of side effects. Thus, viable alternatives to the use of these drugs would be advantageous. In this study, we have analyzed the anti-inflammatory profile of three synthetic peptides first identified in skin secretion of the tree frog Hypsiboas raniceps. Structural characterization was performed using NMR spectroscopy and Mass Spectrometry, and the peptides were tested in vitro in RAW 264.7 cells and in vivo in Balb/c mice for their functional properties. The samples did not show a significant antimicrobial profile. NMR spectroscopy indicated that AC12 (ACFLTRLGTYVC) has a disulfide bond between C2 and C11 and a beta-sheet-turn-beta-sheet conformation in aqueous solution. This peptide showed no cytotoxic effect in mammalian cells and it was the most effective in reducing anti-inflammatory markers, such as NO, TNF-alpha and IL-12. Peptide DK16 (DKERPICSNTFRGRKC) demonstrated anti-inflammatory properties in vitro, while RC11 (RCFRRRGKLTC) significantly altered the cell viability in RAW 264.7 but was shown to be safe in Balb/c erythrocytes. Our results indicate that, of the three peptides studied, AC12 is the most efficient in reducing anti-inflammatory markers, and it could be a potential agent for the treatment of inflammatory diseases.
Host-defense peptides AC12, DK16 and RC11 with immunomodulatory activity isolated from Hypsiboas raniceps skin secretion.,Popov CSFC, Magalhaes BS, Goodfellow BJ, Bocca AL, Pereira DM, Andrade PB, Valentao P, Pereira PJB, Rodrigues JE, de Holanda Veloso PH Junior, Rezende TMB Peptides. 2019 Jan 2;113:11-21. doi: 10.1016/j.peptides.2018.12.007. PMID:30610885[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Popov CSFC, Magalhaes BS, Goodfellow BJ, Bocca AL, Pereira DM, Andrade PB, Valentao P, Pereira PJB, Rodrigues JE, de Holanda Veloso PH Junior, Rezende TMB. Host-defense peptides AC12, DK16 and RC11 with immunomodulatory activity isolated from Hypsiboas raniceps skin secretion. Peptides. 2019 Jan 2;113:11-21. doi: 10.1016/j.peptides.2018.12.007. PMID:30610885 doi:http://dx.doi.org/10.1016/j.peptides.2018.12.007
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