7l1k
From Proteopedia
(Difference between revisions)
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| - | ==== | + | ==Cryo-EM structure of S. Pombe NatC complex with a Bisubstrate inhibitor and inositol hexaphosphate== |
| - | <StructureSection load='7l1k' size='340' side='right'caption='[[7l1k]]' scene=''> | + | <StructureSection load='7l1k' size='340' side='right'caption='[[7l1k]], [[Resolution|resolution]] 3.16Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7l1k]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7L1K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7L1K FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7l1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7l1k OCA], [https://pdbe.org/7l1k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7l1k RCSB], [https://www.ebi.ac.uk/pdbsum/7l1k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7l1k ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.16Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMC:CARBOXYMETHYL+COENZYME+*A'>CMC</scene>, <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7l1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7l1k OCA], [https://pdbe.org/7l1k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7l1k RCSB], [https://www.ebi.ac.uk/pdbsum/7l1k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7l1k ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/NAA38_SCHPO NAA38_SCHPO] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Protein N-terminal acetylation is predominantly a ribosome-associated modification, with NatA-E serving as the major enzymes. NatC is the most unusual of these enzymes, containing one Naa30 catalytic subunit and two auxiliary subunits, Naa35 and Naa38; and substrate selectivity profile that overlaps with NatE. Here, we report the cryoelectron microscopy structure of S. pombe NatC with a NatE/C-type bisubstrate analog and inositol hexaphosphate (IP(6)), and associated biochemistry studies. We find that the presence of three subunits is a prerequisite for normal NatC acetylation activity in yeast and that IP(6) binds tightly to NatC to stabilize the complex. We also describe the molecular basis for IP(6)-mediated NatC complex stabilization and the overlapping yet distinct substrate profiles of NatC and NatE. | ||
| + | |||
| + | Molecular mechanism of N-terminal acetylation by the ternary NatC complex.,Deng S, Gottlieb L, Pan B, Supplee J, Wei X, Petersson EJ, Marmorstein R Structure. 2021 Oct 7;29(10):1094-1104.e4. doi: 10.1016/j.str.2021.05.003. Epub , 2021 May 20. PMID:34019809<ref>PMID:34019809</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7l1k" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Schizosaccharomyces pombe 972h-]] |
| + | [[Category: Deng S]] | ||
| + | [[Category: Marmorstein R]] | ||
Current revision
Cryo-EM structure of S. Pombe NatC complex with a Bisubstrate inhibitor and inositol hexaphosphate
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