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| | <StructureSection load='6zd6' size='340' side='right'caption='[[6zd6]], [[Resolution|resolution]] 2.64Å' scene=''> | | <StructureSection load='6zd6' size='340' side='right'caption='[[6zd6]], [[Resolution|resolution]] 2.64Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6zd6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cantt Cantt]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZD6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZD6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6zd6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_tropicalis_MYA-3404 Candida tropicalis MYA-3404]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZD6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZD6 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CTRG_04008 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=294747 CANTT])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.64Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/RNA-directed_DNA_polymerase RNA-directed DNA polymerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.49 2.7.7.49] </span></td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zd6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zd6 OCA], [https://pdbe.org/6zd6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zd6 RCSB], [https://www.ebi.ac.uk/pdbsum/6zd6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zd6 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zd6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zd6 OCA], [https://pdbe.org/6zd6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zd6 RCSB], [https://www.ebi.ac.uk/pdbsum/6zd6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zd6 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/C5MCQ7_CANTT C5MCQ7_CANTT]] Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme.[RuleBase:RU365061]
| + | [https://www.uniprot.org/uniprot/C5MCQ7_CANTT C5MCQ7_CANTT] Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme.[RuleBase:RU365061] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 6zd6" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 6zd6" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Telomerase 3D structures|Telomerase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Cantt]] | + | [[Category: Candida tropicalis MYA-3404]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: RNA-directed DNA polymerase]]
| + | [[Category: Auguin D]] |
| - | [[Category: Auguin, D]] | + | [[Category: Chen WF]] |
| - | [[Category: Chen, W F]] | + | [[Category: Rety S]] |
| - | [[Category: Rety, S]] | + | [[Category: Xi XG]] |
| - | [[Category: Xi, X G]] | + | [[Category: Zhai L]] |
| - | [[Category: Zhai, L]] | + | |
| - | [[Category: Catalytic core]]
| + | |
| - | [[Category: Replication]]
| + | |
| - | [[Category: Rna binding]]
| + | |
| - | [[Category: Telomerase]]
| + | |
| - | [[Category: Telomere]]
| + | |
| Structural highlights
Function
C5MCQ7_CANTT Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme.[RuleBase:RU365061]
Publication Abstract from PubMed
Telomerase plays critical roles in cellular aging, in the emergence and/or development of cancer, and in the capacity for stem-cell renewal, consists of a catalytic telomerase reverse transcriptase (TERT) and a template-encoding RNA (TER). TERs from diverse organisms contain two conserved structural elements: the template-pseudoknot (T-PK) and a helical three-way junction (TWJ). Species-specific features of the structure and function of telomerase make obtaining a more in-depth understanding of the molecular mechanism of telomerase particularly important. Here, we report the first structural studies of N-terminally truncated TERTs from Candida albicans and Candida tropicalis in apo form and complexed with their respective TWJs in several conformations. We found that Candida TERT proteins perform only one round of telomere addition in the presence or absence of PK/TWJ and display standard reverse transcriptase activity. The C-terminal domain adopts at least two extreme conformations and undergoes conformational interconversion, which regulates the catalytic activity. Most importantly, we identified a conserved tertiary structural motif, called the U-motif, which interacts with the reverse transcriptase domain and is crucial for catalytic activity. Together these results shed new light on the structure and mechanics of fungal TERTs, which show common TERT characteristics, but also display species-specific features.
Crystal structures of N-terminally truncated telomerase reverse transcriptase from fungidouble dagger.,Zhai LT, Rety S, Chen WF, Song ZY, Auguin D, Sun B, Dou SX, Xi XG Nucleic Acids Res. 2021 Apr 15. pii: 6226674. doi: 10.1093/nar/gkab261. PMID:33856462[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Zhai LT, Rety S, Chen WF, Song ZY, Auguin D, Sun B, Dou SX, Xi XG. Crystal structures of N-terminally truncated telomerase reverse transcriptase from fungidouble dagger. Nucleic Acids Res. 2021 Apr 15. pii: 6226674. doi: 10.1093/nar/gkab261. PMID:33856462 doi:http://dx.doi.org/10.1093/nar/gkab261
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