1ees

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{{STRUCTURE_1ees| PDB=1ees | SCENE= }}
{{STRUCTURE_1ees| PDB=1ees | SCENE= }}
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'''SOLUTION STRUCTURE OF CDC42HS COMPLEXED WITH A PEPTIDE DERIVED FROM P-21 ACTIVATED KINASE, NMR, 20 STRUCTURES'''
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===SOLUTION STRUCTURE OF CDC42HS COMPLEXED WITH A PEPTIDE DERIVED FROM P-21 ACTIVATED KINASE, NMR, 20 STRUCTURES===
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==Overview==
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Cdc42Hs is a member of the Ras superfamily of GTPases and initiates a cascade that begins with the activation of several kinases, including p21-activated kinase (PAK). We have previously used a 46 amino acid fragment of PAK (PBD46) to define the binding surface on Cdc42Hs [Guo et al. (1998) Biochemistry 37, 14030-14037]. Here we describe the three-dimensional solution structure of the Cdc42Hs. GMPPCP-PBD46 complex. Heteronuclear NMR methods were used to assign resonances in the complex, and approximately 2400 distance and dihedral restraints were used to calculate a set of 20 structures using a combination of distance geometry, simulated annealing, and chemical shift and Ramachandran refinement. The overall structure of Cdc42Hs in the complex differs from the uncomplexed structure in two major aspects: (1) the first alpha helix is reoriented to accommodate the binding of the peptide and (2) the regions corresponding to switch I and switch II are less disordered. As suggested by our previous work (Guo et al., 1998) and similar to the complex between Cdc42Hs and fACK [Mott et al. (1999) Nature 399, 384-388], PBD46 forms an intermolecular beta-sheet with beta2 of Cdc42Hs and contacts both switch I and switch II. The extensive binding surface between PBD46 and Cdc42Hs can account for both the high affinity of the complex and the inhibition by PBD46 of GTP hydrolysis.
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{{ABSTRACT_PUBMED_10747784}}
==About this Structure==
==About this Structure==
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1EES is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EES OCA].
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1EES is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EES OCA].
==Reference==
==Reference==
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[[Category: Sutcliffe, M J.]]
[[Category: Sutcliffe, M J.]]
[[Category: Protein-protein complex]]
[[Category: Protein-protein complex]]
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Revision as of 21:35, 30 June 2008

Image:1ees.png

Template:STRUCTURE 1ees

SOLUTION STRUCTURE OF CDC42HS COMPLEXED WITH A PEPTIDE DERIVED FROM P-21 ACTIVATED KINASE, NMR, 20 STRUCTURES

Template:ABSTRACT PUBMED 10747784

About this Structure

1EES is a Protein complex structure of sequences from Homo sapiens and Mus musculus. Full experimental information is available from OCA.

Reference

Structure of the complex of Cdc42Hs with a peptide derived from P-21 activated kinase., Gizachew D, Guo W, Chohan KK, Sutcliffe MJ, Oswald RE, Biochemistry. 2000 Apr 11;39(14):3963-71. PMID:10747784

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