7dft
From Proteopedia
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==Crystal structure of Xanthomonas oryzae ClpP== | ==Crystal structure of Xanthomonas oryzae ClpP== | ||
| - | <StructureSection load='7dft' size='340' side='right'caption='[[7dft]]' scene=''> | + | <StructureSection load='7dft' size='340' side='right'caption='[[7dft]], [[Resolution|resolution]] 1.80Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DFT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DFT FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7dft]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/"pseudomonas_oryzae"_uyeda_and_ishiyama_in_ishiyama_1926 "pseudomonas oryzae" uyeda and ishiyama in ishiyama 1926]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DFT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DFT FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dft OCA], [https://pdbe.org/7dft PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dft RCSB], [https://www.ebi.ac.uk/pdbsum/7dft PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dft ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">clpP, ADT25_23080, ADT27_15185, EYR26_04825 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=347 "Pseudomonas oryzae" Uyeda and Ishiyama in Ishiyama 1926])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Endopeptidase_Clp Endopeptidase Clp], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.92 3.4.21.92] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dft OCA], [https://pdbe.org/7dft PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dft RCSB], [https://www.ebi.ac.uk/pdbsum/7dft PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dft ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/A0A0M1K022_9XANT A0A0M1K022_9XANT]] Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins.[HAMAP-Rule:MF_00444][RuleBase:RU000550] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Rice bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is considered a destructive plant bacterial disease. The looming crisis of antibiotic resistance necessitates the discovery of antibiotics with new modes of action. Activated caseinolytic protease P (ClpP) can degrade bacterial FtsZ proteins that are essential for cell division; thus, we hypothesized that small-molecule-induced dysregulation of XooClpP may result in degradation of XooFtsZ to treat leaf blight diseases. In this work, we have determined the crystal structures of XooClpP, and its mutant bound with ADEP4, which revealed the action modes of XooClpP assemblies and XooFtsZ degradation by dysregulated XooClpP in the presence of small-molecule activators, such as ONC212 and ADEP4. Additionally, an antibacterial assessment demonstrated that ONC212 displays excellent activity against Xoo and prevents rice bacterial leaf blight in vivo. Thus, these unique antibacterial effects of small-molecule activators of XooClpP represent a potential strategy for the development of agricultural antibiotics by targeting bacterial ClpP. | ||
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| + | Dysregulation of ClpP by Small-Molecule Activators Used Against Xanthomonas oryzae pv. oryzae Infections.,Yang T, Zhang T, Zhou X, Wang P, Gan J, Song B, Yang S, Yang CG J Agric Food Chem. 2021 Jul 14;69(27):7545-7553. doi: 10.1021/acs.jafc.1c01470., Epub 2021 Jul 3. PMID:34218658<ref>PMID:34218658</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7dft" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Pseudomonas oryzae uyeda and ishiyama in ishiyama 1926]] | ||
| + | [[Category: Endopeptidase Clp]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Yang C | + | [[Category: Yang, C G]] |
| - | [[Category: Yang T]] | + | [[Category: Yang, T]] |
| + | [[Category: Caseinolytic protease p]] | ||
| + | [[Category: Clp protease]] | ||
| + | [[Category: Hydrolase]] | ||
Revision as of 10:47, 28 July 2021
Crystal structure of Xanthomonas oryzae ClpP
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