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1aw7

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Q136A MUTANT OF TOXIC SHOCK SYNDROME TOXIN-1 FROM S. AUREUS

Structural highlights

1aw7 is a 4 chain structure with sequence from Staphylococcus aureus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.95Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TSST_STAAU Responsible for the symptoms of toxic shock syndrome.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The three-dimensional structures of five mutants of toxic shock syndrome toxin-1 (TSST-1) have been determined. These mutations are in the long central alpha helix and are useful in mapping portions of TSST-1 involved in superantigenicity and lethality. The T128A, H135A, Q139K, and I140T mutations appear to reduce superantigenicity by altering the properties of the T-cell receptor interaction surface. The Q136A mutation is at a largely buried site and causes a dramatic change in the conformation of the beta7-beta9 loop which covers the back of the central alpha helix. As this mutation has the unique ability to reduce the toxin's lethality in rabbits while retaining its superantigenicity, it raises the possibility that this rear loop mediates the ability of TSST-1 to induce lethality and suggests a route for producing nonlethal toxins for therapeutic development.

Structures of five mutants of toxic shock syndrome toxin-1 with reduced biological activity.,Earhart CA, Mitchell DT, Murray DL, Pinheiro DM, Matsumura M, Schlievert PM, Ohlendorf DH Biochemistry. 1998 May 19;37(20):7194-202. PMID:9585531^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Earhart CA, Mitchell DT, Murray DL, Pinheiro DM, Matsumura M, Schlievert PM, Ohlendorf DH. Structures of five mutants of toxic shock syndrome toxin-1 with reduced biological activity. Biochemistry. 1998 May 19;37(20):7194-202. PMID:9585531 doi:10.1021/bi9721896

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1aw7"

@@ Line 3: / Line 3: @@
 <StructureSection load='1aw7' size='340' side='right'caption='[[1aw7]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1aw7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AW7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AW7 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1aw7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AW7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AW7 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1aw7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aw7 OCA], [https://pdbe.org/1aw7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1aw7 RCSB], [https://www.ebi.ac.uk/pdbsum/1aw7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1aw7 ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1aw7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aw7 OCA], [https://pdbe.org/1aw7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1aw7 RCSB], [https://www.ebi.ac.uk/pdbsum/1aw7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1aw7 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/TSST_STAAU TSST_STAAU]] Responsible for the symptoms of toxic shock syndrome.
+[https://www.uniprot.org/uniprot/TSST_STAAU TSST_STAAU] Responsible for the symptoms of toxic shock syndrome.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 32: / Line 33: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Earhart, C A]]
+[[Category: Staphylococcus aureus]]
-[[Category: Matsumura, M]]
+[[Category: Earhart CA]]
-[[Category: Mitchell, D T]]
+[[Category: Matsumura M]]
-[[Category: Murray, D L]]
+[[Category: Mitchell DT]]
-[[Category: Ohlendorf, D H]]
+[[Category: Murray DL]]
-[[Category: Pinheiro, D M]]
+[[Category: Ohlendorf DH]]
-[[Category: Schlievert, P M]]
+[[Category: Pinheiro DM]]
-[[Category: Superantigen]]
+[[Category: Schlievert PM]]
-[[Category: Toxin]]

1aw7

From Proteopedia

Current revision

Q136A MUTANT OF TOXIC SHOCK SYNDROME TOXIN-1 FROM S. AUREUS

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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