1avf
From Proteopedia
(New page: 200px<br /> <applet load="1avf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1avf, resolution 2.36Å" /> '''ACTIVATION INTERMED...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1avf.gif|left|200px]]<br /> | + | [[Image:1avf.gif|left|200px]]<br /><applet load="1avf" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1avf" size=" | + | |
caption="1avf, resolution 2.36Å" /> | caption="1avf, resolution 2.36Å" /> | ||
'''ACTIVATION INTERMEDIATE 2 OF HUMAN GASTRICSIN FROM HUMAN STOMACH'''<br /> | '''ACTIVATION INTERMEDIATE 2 OF HUMAN GASTRICSIN FROM HUMAN STOMACH'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of an activation intermediate of human gastricsin | + | The crystal structure of an activation intermediate of human gastricsin has been determined at 2.4 A resolution. The human digestive enzyme gastricsin (pepsin C) is an aspartic proteinase that is synthesized as the inactive precursor (zymogen) progastricsin (pepsinogen C or hPGC). In the zymogen, a positively-charged N-terminal prosegment of 43 residues (Ala 1p-Leu 43p; the suffix 'p' refers to the prosegment) sterically prevents the approach of a substrate to the active site. Zymogen conversion occurs in an autocatalytic and stepwise fashion at low pH through the formation of intermediates. The structure of the non-covalent complex of a partially-cleaved peptide of the prosegment (Ala 1p-Phe 26p) with mature gastricsin (Ser 1-Ala 329) suggests an activation pathway that may be common to all gastric aspartic proteinases. |
==Disease== | ==Disease== | ||
| Line 11: | Line 10: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1AVF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with NA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Gastricsin Gastricsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.3 3.4.23.3] Full crystallographic information is available from [http:// | + | 1AVF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=NA:'>NA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Gastricsin Gastricsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.3 3.4.23.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AVF OCA]. |
==Reference== | ==Reference== | ||
| Line 18: | Line 17: | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Cherney, M | + | [[Category: Cherney, M M.]] |
| - | [[Category: James, M | + | [[Category: James, M N.G.]] |
| - | [[Category: Khan, A | + | [[Category: Khan, A R.]] |
| - | [[Category: Tarasova, N | + | [[Category: Tarasova, N I.]] |
[[Category: NA]] | [[Category: NA]] | ||
[[Category: acid]] | [[Category: acid]] | ||
| Line 30: | Line 29: | ||
[[Category: intermediate]] | [[Category: intermediate]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:48:39 2008'' |
Revision as of 09:48, 21 February 2008
|
ACTIVATION INTERMEDIATE 2 OF HUMAN GASTRICSIN FROM HUMAN STOMACH
Contents |
Overview
The crystal structure of an activation intermediate of human gastricsin has been determined at 2.4 A resolution. The human digestive enzyme gastricsin (pepsin C) is an aspartic proteinase that is synthesized as the inactive precursor (zymogen) progastricsin (pepsinogen C or hPGC). In the zymogen, a positively-charged N-terminal prosegment of 43 residues (Ala 1p-Leu 43p; the suffix 'p' refers to the prosegment) sterically prevents the approach of a substrate to the active site. Zymogen conversion occurs in an autocatalytic and stepwise fashion at low pH through the formation of intermediates. The structure of the non-covalent complex of a partially-cleaved peptide of the prosegment (Ala 1p-Phe 26p) with mature gastricsin (Ser 1-Ala 329) suggests an activation pathway that may be common to all gastric aspartic proteinases.
Disease
Known disease associated with this structure: Obesity, variation in OMIM:[608886]
About this Structure
1AVF is a Protein complex structure of sequences from Homo sapiens with as ligand. Active as Gastricsin, with EC number 3.4.23.3 Full crystallographic information is available from OCA.
Reference
Structural characterization of activation 'intermediate 2' on the pathway to human gastricsin., Khan AR, Cherney MM, Tarasova NI, James MN, Nat Struct Biol. 1997 Dec;4(12):1010-5. PMID:9406551
Page seeded by OCA on Thu Feb 21 11:48:39 2008
