1mow

<table><tr><td colspan='2'>[[1mow]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_35582_[[desulfurococcus_mobilis]] Atcc 35582 [[desulfurococcus mobilis]]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MOW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MOW FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>

[[https://www.uniprot.org/uniprot/DMO1_DESMO DMO1_DESMO]] Endonuclease involved in intron homing. Recognizes a recognizes up to 20 bp of DNA in the 23S rRNA gene intron. It has a slow turnover rate and cuts the coding strand with a slight preference over the non-coding strand.

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== Publication Abstract from PubMed ==

We have generated an artificial highly specific endonuclease by fusing domains of homing endonucleases I-DmoI and I-CreI and creating a new 1400 A(2) protein interface between these domains. Protein engineering was accomplished by combining computational redesign and an in vivo protein-folding screen. The resulting enzyme, E-DreI (Engineered I-DmoI/I-CreI), binds a long chimeric DNA target site with nanomolar affinity, cleaving it precisely at a rate equivalent to its natural parents. The structure of an E-DreI/DNA complex demonstrates the accuracy of the protein interface redesign algorithm and reveals how catalytic function is maintained during the creation of the new endonuclease. These results indicate that it may be possible to generate novel highly specific DNA binding proteins from homing endonucleases.

Design, activity, and structure of a highly specific artificial endonuclease.,Chevalier BS, Kortemme T, Chadsey MS, Baker D, Monnat RJ, Stoddard BL Mol Cell. 2002 Oct;10(4):895-905. PMID:12419232<ref>PMID:12419232</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 1mow" style="background-color:#fffaf0;"></div>

== References ==

<references/>

[[Category: Baker, D]]

[[Category: Chadsey, M S]]

[[Category: Chevalier, B S]]

[[Category: Kortemme, T]]

[[Category: Monnat, R J]]

[[Category: Stoddard, B L]]

[[Category: Laglidadg]]