1axi
From Proteopedia
(New page: 200px<br /> <applet load="1axi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1axi, resolution 2.1Å" /> '''STRUCTURAL PLASTICIT...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1axi.gif|left|200px]]<br /> | + | [[Image:1axi.gif|left|200px]]<br /><applet load="1axi" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1axi" size=" | + | |
caption="1axi, resolution 2.1Å" /> | caption="1axi, resolution 2.1Å" /> | ||
'''STRUCTURAL PLASTICITY AT THE HGH:HGHBP INTERFACE'''<br /> | '''STRUCTURAL PLASTICITY AT THE HGH:HGHBP INTERFACE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Remodeling of the interface between human growth hormone (hGH) and the | + | Remodeling of the interface between human growth hormone (hGH) and the extracellular domain of its receptor was studied by deleting a critical tryptophan residue (at position 104) in the receptor, creating a large cavity, and selecting a pentamutant of hGH by phage display that fills the cavity and largely restores binding affinity. A 2.1 A resolution x-ray structure of the mutant complex showed that the receptor cavity was filled by selected hydrophobic mutations of hGH. Large structural rearrangements occurred in the interface at sites that were distant from the mutations. Such plasticity may be a means for protein-protein interfaces to adapt to mutations as they coevolve. |
==Disease== | ==Disease== | ||
| Line 11: | Line 10: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1AXI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1AXI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AXI OCA]. |
==Reference== | ==Reference== | ||
| Line 19: | Line 18: | ||
[[Category: Atwell, S.]] | [[Category: Atwell, S.]] | ||
[[Category: Ultsch, M.]] | [[Category: Ultsch, M.]] | ||
| - | [[Category: Vos, A | + | [[Category: Vos, A M.De.]] |
| - | [[Category: Wells, J | + | [[Category: Wells, J A.]] |
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: complex (hormone/receptor)]] | [[Category: complex (hormone/receptor)]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:49:16 2008'' |
Revision as of 09:49, 21 February 2008
|
STRUCTURAL PLASTICITY AT THE HGH:HGHBP INTERFACE
Contents |
Overview
Remodeling of the interface between human growth hormone (hGH) and the extracellular domain of its receptor was studied by deleting a critical tryptophan residue (at position 104) in the receptor, creating a large cavity, and selecting a pentamutant of hGH by phage display that fills the cavity and largely restores binding affinity. A 2.1 A resolution x-ray structure of the mutant complex showed that the receptor cavity was filled by selected hydrophobic mutations of hGH. Large structural rearrangements occurred in the interface at sites that were distant from the mutations. Such plasticity may be a means for protein-protein interfaces to adapt to mutations as they coevolve.
Disease
Known diseases associated with this structure: Growth hormone deficiency OMIM:[139250], Growth hormone deficiency, isolated, type IA OMIM:[139250], Growth hormone deficiency, isolated, type IB OMIM:[139250], Growth hormone deficiency, isolated, type II OMIM:[139250], Increased responsiveness to growth hormone OMIM:[600946], Kowarski syndrome OMIM:[139250], Laron dwarfism OMIM:[600946], Short stature, autosomal dominant, with normal serum growth hormone binding protein OMIM:[600946], Short stature, familial OMIM:[139250], Short stature, idiopathic OMIM:[600946]
About this Structure
1AXI is a Protein complex structure of sequences from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
Structural plasticity in a remodeled protein-protein interface., Atwell S, Ultsch M, De Vos AM, Wells JA, Science. 1997 Nov 7;278(5340):1125-8. PMID:9353194
Page seeded by OCA on Thu Feb 21 11:49:16 2008
