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6xjj
From Proteopedia
(Difference between revisions)
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==Structure of non-heme iron enzyme TropC: Radical tropolone biosynthesis== | ==Structure of non-heme iron enzyme TropC: Radical tropolone biosynthesis== | ||
| - | <StructureSection load='6xjj' size='340' side='right'caption='[[6xjj]]' scene=''> | + | <StructureSection load='6xjj' size='340' side='right'caption='[[6xjj]], [[Resolution|resolution]] 2.70Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XJJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XJJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6xjj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Talaromyces_stipitatus_ATCC_10500 Talaromyces stipitatus ATCC 10500]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XJJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XJJ FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xjj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xjj OCA], [https://pdbe.org/6xjj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xjj RCSB], [https://www.ebi.ac.uk/pdbsum/6xjj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xjj ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=YT3:YTTRIUM+(III)+ION'>YT3</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xjj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xjj OCA], [https://pdbe.org/6xjj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xjj RCSB], [https://www.ebi.ac.uk/pdbsum/6xjj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xjj ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/TROPC_TALSN TROPC_TALSN] 2-oxoglutarate-dependent dioxygenase; part of the gene cluster that mediates the biosynthesis of the tropolone class of fungal maleic anhydrides (PubMed:22508998, PubMed:24863423). The pathway begins with the synthesis of 3-methylorcinaldehyde by the non-reducing polyketide synthase (PKS) tropA (PubMed:22508998). 3-methylorcinaldehyde is the substrate for the FAD-dependent monooxygenase tropB to yield a dearomatized hydroxycyclohexadione (PubMed:22508998, PubMed:24863423). The 2-oxoglutarate-dependent dioxygenase tropC then performs the oxidative ring expansion to provide the first tropolone metabolite stipitaldehyde (PubMed:22508998, PubMed:24863423). Trop D converts stipitaldehyde into stipitacetal which is in turn converted to stipitalide by the short-chain dehydrogenase/reductase tropE (PubMed:24863423). The next steps involve tropF, tropG, tropH, tropI and tropJ to form successive tropolone maleic anhydrides including stipitaldehydic, stipitatonic and stipitatic acids (PubMed:24863423).<ref>PMID:22508998</ref> <ref>PMID:24863423</ref> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Talaromyces stipitatus ATCC 10500]] | ||
[[Category: Doyon TJ]] | [[Category: Doyon TJ]] | ||
[[Category: Koutmos M]] | [[Category: Koutmos M]] | ||
[[Category: Mallik L]] | [[Category: Mallik L]] | ||
[[Category: Narayan ARH]] | [[Category: Narayan ARH]] | ||
Current revision
Structure of non-heme iron enzyme TropC: Radical tropolone biosynthesis
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