7od4
From Proteopedia
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| - | ==Hepatitis B core protein== | + | ==Hepatitis B core protein.== |
| - | <StructureSection load='7od4' size='340' side='right'caption='[[7od4]]' scene=''> | + | <StructureSection load='7od4' size='340' side='right'caption='[[7od4]], [[Resolution|resolution]] 2.80Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OD4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OD4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7od4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Hbv Hbv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OD4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OD4 FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7od4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7od4 OCA], [https://pdbe.org/7od4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7od4 RCSB], [https://www.ebi.ac.uk/pdbsum/7od4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7od4 ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7od4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7od4 OCA], [https://pdbe.org/7od4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7od4 RCSB], [https://www.ebi.ac.uk/pdbsum/7od4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7od4 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/W6CP35_HBV W6CP35_HBV]] May regulate immune response to the intracellular capsid in acting as a T-cell tolerogen, by having an immunoregulatory effect which prevents destruction of infected cells by cytotoxic T-cells.[RuleBase:RU361253] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Hepatitis B virus is a major human pathogen, which forms enveloped virus particles. During viral maturation, membrane-bound hepatitis B surface proteins package hepatitis B core protein capsids. This process is intercepted by certain peptides with an "LLGRMKG" motif that binds to the capsids at the tips of dimeric spikes. With microcalorimetry, electron cryo microscopy and peptide microarray-based screens, we have characterized the structural and thermodynamic properties of peptide binding to hepatitis B core protein capsids with different secretion phenotypes. The peptide "GSLLGRMKGA" binds weakly to hepatitis B core protein capsids and mutant capsids with a premature (F97L) or low-secretion phenotype (L60V and P5T). With electron cryo microscopy, we provide novel structures for L60V and P5T and demonstrate that binding occurs at the tips of the spikes at the dimer interface, splaying the helices apart independent of the secretion phenotype. Peptide array screening identifies "SLLGRM" as the core binding motif. This shortened motif binds only to one of the two spikes in the asymmetric unit of the capsid and induces a much smaller conformational change. Altogether, these comprehensive studies suggest that the tips of the spikes act as an autonomous binding platform that is unaffected by mutations that affect secretion phenotypes. | ||
| + | |||
| + | Conformational Plasticity of Hepatitis B Core Protein Spikes Promotes Peptide Binding Independent of the Secretion Phenotype.,Makbul C, Khayenko V, Maric HM, Bottcher B Microorganisms. 2021 Apr 29;9(5). pii: microorganisms9050956. doi:, 10.3390/microorganisms9050956. PMID:33946808<ref>PMID:33946808</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7od4" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Hbv]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Bottcher B]] | + | [[Category: Bottcher, B]] |
| - | [[Category: Makbul C]] | + | [[Category: Makbul, C]] |
| + | [[Category: Hepatitis b core protein]] | ||
| + | [[Category: Virus like particle]] | ||
Revision as of 12:37, 9 June 2021
Hepatitis B core protein.
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