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| | <StructureSection load='4iv3' size='340' side='right'caption='[[4iv3]], [[Resolution|resolution]] 2.90Å' scene=''> | | <StructureSection load='4iv3' size='340' side='right'caption='[[4iv3]], [[Resolution|resolution]] 2.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4iv3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aphthovirus_a Aphthovirus a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IV3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IV3 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4iv3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Foot-and-mouth_disease_virus_A Foot-and-mouth disease virus A]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IV3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IV3 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4iv1|4iv1]], [[4gh4|4gh4]]</div></td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4iv3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iv3 OCA], [https://pdbe.org/4iv3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4iv3 RCSB], [https://www.ebi.ac.uk/pdbsum/4iv3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4iv3 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4iv3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iv3 OCA], [https://pdbe.org/4iv3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4iv3 RCSB], [https://www.ebi.ac.uk/pdbsum/4iv3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4iv3 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/Q6PN23_9PICO Q6PN23_9PICO]] Protein 3C is a cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind co-operatively to the protease (By similarity).[SAAS:SAAS000199_004_042266] RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals (By similarity).[SAAS:SAAS000199_004_010047]
| + | [https://www.uniprot.org/uniprot/Q6PN23_9PICO Q6PN23_9PICO] Protein 3C is a cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind co-operatively to the protease (By similarity).[SAAS:SAAS000199_004_042266] RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals (By similarity).[SAAS:SAAS000199_004_010047] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Aphthovirus a]] | + | [[Category: Foot-and-mouth disease virus A]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Burman, A]] | + | [[Category: Burman A]] |
| - | [[Category: Charleston, B]] | + | [[Category: Charleston B]] |
| - | [[Category: Fry, E E]] | + | [[Category: Fry EE]] |
| - | [[Category: Jackson, T]] | + | [[Category: Jackson T]] |
| - | [[Category: Jones, I M]] | + | [[Category: Jones IM]] |
| - | [[Category: Kotecha, A]] | + | [[Category: Kotecha A]] |
| - | [[Category: Loureiro, S]] | + | [[Category: Loureiro S]] |
| - | [[Category: Porta, C]] | + | [[Category: Porta C]] |
| - | [[Category: Ren, J]] | + | [[Category: Ren J]] |
| - | [[Category: Stuart, D I]] | + | [[Category: Stuart DI]] |
| - | [[Category: Apthovirus]]
| + | |
| - | [[Category: Capsid]]
| + | |
| - | [[Category: Icosahedral virus]]
| + | |
| - | [[Category: Picornavirus]]
| + | |
| - | [[Category: Vaccine]]
| + | |
| - | [[Category: Virus]]
| + | |
| Structural highlights
Function
Q6PN23_9PICO Protein 3C is a cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind co-operatively to the protease (By similarity).[SAAS:SAAS000199_004_042266] RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals (By similarity).[SAAS:SAAS000199_004_010047]
Publication Abstract from PubMed
Foot-and-mouth disease remains a major plague of livestock and outbreaks are often economically catastrophic. Current inactivated virus vaccines require expensive high containment facilities for their production and maintenance of a cold-chain for their activity. We have addressed both of these major drawbacks. Firstly we have developed methods to efficiently express recombinant empty capsids. Expression constructs aimed at lowering the levels and activity of the viral protease required for the cleavage of the capsid protein precursor were used; this enabled the synthesis of empty A-serotype capsids in eukaryotic cells at levels potentially attractive to industry using both vaccinia virus and baculovirus driven expression. Secondly we have enhanced capsid stability by incorporating a rationally designed mutation, and shown by X-ray crystallography that stabilised and wild-type empty capsids have essentially the same structure as intact virus. Cattle vaccinated with recombinant capsids showed sustained virus neutralisation titres and protection from challenge 34 weeks after immunization. This approach to vaccine antigen production has several potential advantages over current technologies by reducing production costs, eliminating the risk of infectivity and enhancing the temperature stability of the product. Similar strategies that will optimize host cell viability during expression of a foreign toxic gene and/or improve capsid stability could allow the production of safe vaccines for other pathogenic picornaviruses of humans and animals.
Rational engineering of recombinant picornavirus capsids to produce safe, protective vaccine antigen.,Porta C, Kotecha A, Burman A, Jackson T, Ren J, Loureiro S, Jones IM, Fry EE, Stuart DI, Charleston B PLoS Pathog. 2013 Mar;9(3):e1003255. doi: 10.1371/journal.ppat.1003255. Epub 2013, Mar 27. PMID:23544011[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Porta C, Kotecha A, Burman A, Jackson T, Ren J, Loureiro S, Jones IM, Fry EE, Stuart DI, Charleston B. Rational engineering of recombinant picornavirus capsids to produce safe, protective vaccine antigen. PLoS Pathog. 2013 Mar;9(3):e1003255. doi: 10.1371/journal.ppat.1003255. Epub 2013, Mar 27. PMID:23544011 doi:http://dx.doi.org/10.1371/journal.ppat.1003255
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