1aw6
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==GAL4 (CD), NMR, 24 STRUCTURES== | ==GAL4 (CD), NMR, 24 STRUCTURES== | ||
| - | <StructureSection load='1aw6' size='340' side='right'caption='[[1aw6 | + | <StructureSection load='1aw6' size='340' side='right'caption='[[1aw6]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[1aw6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[1aw6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=125d 125d]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AW6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AW6 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1aw6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aw6 OCA], [https://pdbe.org/1aw6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1aw6 RCSB], [https://www.ebi.ac.uk/pdbsum/1aw6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1aw6 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1aw6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aw6 OCA], [https://pdbe.org/1aw6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1aw6 RCSB], [https://www.ebi.ac.uk/pdbsum/1aw6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1aw6 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | + | [https://www.uniprot.org/uniprot/GAL4_YEAST GAL4_YEAST] This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which code for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes. | |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
| Line 19: | Line 20: | ||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1aw6 ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1aw6 ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | We have refined the solution structure of cadmium-bound GAL4 and present its 15N and 1H NMR assignments. The root-mean-square (rms) deviation to the average structure was 0.4 +/- 0.05 A for backbone atoms, and 0.9 +/- 0.1 A for all heavy atoms. The three-bond heteronuclear 3J(113Cd,1H) coupling constants were found to disobey a Karplus-type relationship, which was attributable to the unusual constraints imposed by the bimetal-thiolate cluster in GAL4. We conclude that the structural parameters that correlate to 3J(113Cd,1H) are complex. | ||
| - | |||
| - | Refined solution structure of the DNA-binding domain of GAL4 and use of 3J(113Cd,1H) in structure determination.,Baleja JD, Thanabal V, Wagner G J Biomol NMR. 1997 Dec;10(4):397-401. PMID:9460244<ref>PMID:9460244</ref> | ||
| - | |||
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 1aw6" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[Gal3-Gal80-Gal4|Gal3-Gal80-Gal4]] | *[[Gal3-Gal80-Gal4|Gal3-Gal80-Gal4]] | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: Atcc 18824]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: | + | [[Category: Baleja JD]] |
| - | [[Category: | + | [[Category: Wagner G]] |
| - | + | ||
Revision as of 15:28, 13 March 2024
GAL4 (CD), NMR, 24 STRUCTURES
| |||||||||||
