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| | ==NMR-based docking model of GrxS14-BolA2 apo-heterodimer from Arabidopsis thaliana== | | ==NMR-based docking model of GrxS14-BolA2 apo-heterodimer from Arabidopsis thaliana== |
| - | <StructureSection load='2mma' size='340' side='right'caption='[[2mma]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''> | + | <StructureSection load='2mma' size='340' side='right'caption='[[2mma]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2mma]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arath Arath]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MMA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MMA FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2mma]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MMA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MMA FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3ipz|3ipz]], [[2mm9|2mm9]]</div></td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mma FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mma OCA], [https://pdbe.org/2mma PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mma RCSB], [https://www.ebi.ac.uk/pdbsum/2mma PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mma ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GRXS14, CXIP1, At3g54900, F28P10.120 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH]), AT5G09830, At5g09830 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mma FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mma OCA], [https://pdbe.org/2mma PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mma RCSB], [https://www.ebi.ac.uk/pdbsum/2mma PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mma ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/GRS14_ARATH GRS14_ARATH]] May only reduce GSH-thiol disulfides, but not protein disulfides (Potential). May protect cells against protein oxidative damage. May regulate CAX cation transporters.<ref>PMID:16829529</ref>
| + | [https://www.uniprot.org/uniprot/GRS14_ARATH GRS14_ARATH] May only reduce GSH-thiol disulfides, but not protein disulfides (Potential). May protect cells against protein oxidative damage. May regulate CAX cation transporters.<ref>PMID:16829529</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Arath]] | + | [[Category: Arabidopsis thaliana]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Couturier, J]] | + | [[Category: Couturier J]] |
| - | [[Category: Didierjean, C]] | + | [[Category: Didierjean C]] |
| - | [[Category: Roret, T]] | + | [[Category: Roret T]] |
| - | [[Category: Rouhier, N]] | + | [[Category: Rouhier N]] |
| - | [[Category: Tsan, P]] | + | [[Category: Tsan P]] |
| - | [[Category: Gene regulation]]
| + | |
| - | [[Category: Stress-responsive protein]]
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| - | [[Category: Transcriptional regulator]]
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| Structural highlights
Function
GRS14_ARATH May only reduce GSH-thiol disulfides, but not protein disulfides (Potential). May protect cells against protein oxidative damage. May regulate CAX cation transporters.[1]
Publication Abstract from PubMed
BolA proteins are defined as stress-responsive transcriptional regulators but they also participate to iron metabolism. Although they can form [2Fe-2S]-containing complexes with monothiol glutaredoxins (Grx), structural details are lacking. Three Arabidopsis thaliana BolA structures were solved. They differ primarily by the size of a loop referred to as the variable [H/C] loop which contains an important cysteine (BolA_C group) or histidine (BolA_H group) residue. From 3D modeling and spectroscopic analyses of A. thaliana GrxS14-BolA1 holo-heterodimer (BolA_H), we provide evidence for the coordination of a Rieske-type [2Fe-2S] cluster. For BolA_C members, the cysteine could replace the histidine as a ligand. NMR interaction experiments using apo-proteins indicate that a completely different heterodimer was formed, involving the nucleic acid binding site of BolA and the C-terminal tail of Grx. The possible biological importance of these complexes is discussed considering the physiological functions previously assigned to BolA and to Grx-BolA or Grx-Grx complexes.
Structural and spectroscopic insights into BolA-glutaredoxin complexes.,Roret T, Tsan P, Couturier J, Zhang B, Johnson MK, Rouhier N, Didierjean C J Biol Chem. 2014 Jul 10. pii: jbc.M114.572701. PMID:25012657[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Cheng NH, Liu JZ, Brock A, Nelson RS, Hirschi KD. AtGRXcp, an Arabidopsis chloroplastic glutaredoxin, is critical for protection against protein oxidative damage. J Biol Chem. 2006 Sep 8;281(36):26280-8. Epub 2006 Jul 7. PMID:16829529 doi:M601354200
- ↑ Roret T, Tsan P, Couturier J, Zhang B, Johnson MK, Rouhier N, Didierjean C. Structural and spectroscopic insights into BolA-glutaredoxin complexes. J Biol Chem. 2014 Jul 10. pii: jbc.M114.572701. PMID:25012657 doi:http://dx.doi.org/10.1074/jbc.M114.572701
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