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| | ==Dynamic binding mode of a synaptotagmin-1-SNARE complex in solution== | | ==Dynamic binding mode of a synaptotagmin-1-SNARE complex in solution== |
| - | <StructureSection load='2n1t' size='340' side='right'caption='[[2n1t]], [[NMR_Ensembles_of_Models | 5 NMR models]]' scene=''> | + | <StructureSection load='2n1t' size='340' side='right'caption='[[2n1t]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2n1t]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat] and [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N1T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N1T FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2n1t]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N1T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N1T FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1sfc|1sfc]], [[1uow|1uow]]</div></td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n1t OCA], [https://pdbe.org/2n1t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n1t RCSB], [https://www.ebi.ac.uk/pdbsum/2n1t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n1t ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Syb2, Vamp2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), Sap, Stx1a ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), SNAP, SNAP25, SNAP-25A ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), SVP65, SYT, SYT1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n1t OCA], [https://pdbe.org/2n1t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n1t RCSB], [https://www.ebi.ac.uk/pdbsum/2n1t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n1t ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/SNP25_HUMAN SNP25_HUMAN]] t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. [[https://www.uniprot.org/uniprot/VAMP2_RAT VAMP2_RAT]] Involved in the targeting and/or fusion of transport vesicles to their target membrane (By similarity). [[https://www.uniprot.org/uniprot/STX1A_RAT STX1A_RAT]] Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm. [[https://www.uniprot.org/uniprot/SYT1_HUMAN SYT1_HUMAN]] May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.
| + | [https://www.uniprot.org/uniprot/VAMP2_RAT VAMP2_RAT] Involved in the targeting and/or fusion of transport vesicles to their target membrane (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Buffalo rat]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Human]]
| + | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bacaj, T]] | + | [[Category: Rattus norvegicus]] |
| - | [[Category: Barlow, N]] | + | [[Category: Bacaj T]] |
| - | [[Category: Bonvin, A]] | + | [[Category: Barlow N]] |
| - | [[Category: Brewer, K]] | + | [[Category: Bonvin A]] |
| - | [[Category: Camilloni, C]] | + | [[Category: Brewer K]] |
| - | [[Category: Cavalli, A]] | + | [[Category: Camilloni C]] |
| - | [[Category: Graham, B]] | + | [[Category: Cavalli A]] |
| - | [[Category: Haussinger, D]] | + | [[Category: Graham B]] |
| - | [[Category: Liu, J]] | + | [[Category: Haussinger D]] |
| - | [[Category: Prinslow, E]] | + | [[Category: Liu J]] |
| - | [[Category: Rizo, J]] | + | [[Category: Prinslow E]] |
| - | [[Category: Seven, A]] | + | [[Category: Rizo J]] |
| - | [[Category: Sudhof, T]] | + | [[Category: Seven A]] |
| - | [[Category: Swarbrick, J]] | + | [[Category: Sudhof T]] |
| - | [[Category: Tomchick, D]] | + | [[Category: Swarbrick J]] |
| - | [[Category: Vendruscolo, M]] | + | [[Category: Tomchick D]] |
| - | [[Category: Voleti, R]] | + | [[Category: Vendruscolo M]] |
| - | [[Category: Xu, J]] | + | [[Category: Voleti R]] |
| - | [[Category: Zhou, A]] | + | [[Category: Xu J]] |
| - | [[Category: Zhou, P]] | + | [[Category: Zhou A]] |
| - | [[Category: C2b domain]]
| + | [[Category: Zhou P]] |
| - | [[Category: Exocytosis]]
| + | |
| - | [[Category: Snap-25]]
| + | |
| - | [[Category: Snap-25a]]
| + | |
| - | [[Category: Snare complex]]
| + | |
| - | [[Category: Synaptobrevin-2]]
| + | |
| - | [[Category: Synaptotagmin-1]]
| + | |
| - | [[Category: Syntaxin-1a]]
| + | |
| Structural highlights
Function
VAMP2_RAT Involved in the targeting and/or fusion of transport vesicles to their target membrane (By similarity).
Publication Abstract from PubMed
Rapid neurotransmitter release depends on the Ca2+ sensor Synaptotagmin-1 (Syt1) and the SNARE complex formed by synaptobrevin, syntaxin-1 and SNAP-25. How Syt1 triggers release has been unclear, partly because elucidating high-resolution structures of Syt1-SNARE complexes has been challenging. An NMR approach based on lanthanide-induced pseudocontact shifts now reveals a dynamic binding mode in which basic residues in the concave side of the Syt1 C2B-domain beta-sandwich interact with a polyacidic region of the SNARE complex formed by syntaxin-1 and SNAP-25. The physiological relevance of this dynamic structural model is supported by mutations in basic residues of Syt1 that markedly impair SNARE-complex binding in vitro and Syt1 function in neurons. Mutations with milder effects on binding have correspondingly milder effects on Syt1 function. Our results support a model whereby dynamic interaction facilitates cooperation between Syt1 and the SNAREs in inducing membrane fusion.
Dynamic binding mode of a Synaptotagmin-1-SNARE complex in solution.,Brewer KD, Bacaj T, Cavalli A, Camilloni C, Swarbrick JD, Liu J, Zhou A, Zhou P, Barlow N, Xu J, Seven AB, Prinslow EA, Voleti R, Haussinger D, Bonvin AM, Tomchick DR, Vendruscolo M, Graham B, Sudhof TC, Rizo J Nat Struct Mol Biol. 2015 Jun 1. doi: 10.1038/nsmb.3035. PMID:26030874[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Brewer KD, Bacaj T, Cavalli A, Camilloni C, Swarbrick JD, Liu J, Zhou A, Zhou P, Barlow N, Xu J, Seven AB, Prinslow EA, Voleti R, Haussinger D, Bonvin AM, Tomchick DR, Vendruscolo M, Graham B, Sudhof TC, Rizo J. Dynamic binding mode of a Synaptotagmin-1-SNARE complex in solution. Nat Struct Mol Biol. 2015 Jun 1. doi: 10.1038/nsmb.3035. PMID:26030874 doi:http://dx.doi.org/10.1038/nsmb.3035
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