1ekm

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1ekm.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1ekm.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1ekm| PDB=1ekm | SCENE= }}
{{STRUCTURE_1ekm| PDB=1ekm | SCENE= }}
-
'''CRYSTAL STRUCTURE AT 2.5 A RESOLUTION OF ZINC-SUBSTITUTED COPPER AMINE OXIDASE OF HANSENULA POLYMORPHA EXPRESSED IN ESCHERICHIA COLI'''
+
===CRYSTAL STRUCTURE AT 2.5 A RESOLUTION OF ZINC-SUBSTITUTED COPPER AMINE OXIDASE OF HANSENULA POLYMORPHA EXPRESSED IN ESCHERICHIA COLI===
-
==Overview==
+
<!--
-
Copper amine oxidases (CAOs) catalyze the two-electron oxidation of primary amines to aldehydes, utilizing molecular oxygen as a terminal electron acceptor. To accomplish this transformation, CAOs utilize two cofactors: a mononuclear copper, and a unique redox cofactor, 2,4,5-trihydroxyphenylalanine quinone (TPQ or TOPA quinone). TPQ is derived via posttranslational modification of a specific tyrosine residue within the protein itself. In this study, the structure of an amine oxidase from Hansenula polymorpha has been solved to 2.5 A resolution, in which the precursor tyrosine is unprocessed to TPQ, and the copper site is occupied by zinc. Significantly, the precursor tyrosine directly ligands the metal, thus providing the closest analogue to date of an intermediate in TPQ production. Besides this result, the rearrangement of other active site residues (relative to the mature enzyme) proposed to be involved in the binding of molecular oxygen may shed light on how CAOs efficiently use their active site to carry out both cofactor formation and catalysis.
+
The line below this paragraph, {{ABSTRACT_PUBMED_10933787}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 10933787 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_10933787}}
==About this Structure==
==About this Structure==
Line 30: Line 34:
[[Category: Amine oxidase]]
[[Category: Amine oxidase]]
[[Category: Quinoprotein]]
[[Category: Quinoprotein]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:13:05 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:54:02 2008''

Revision as of 21:54, 30 June 2008

Image:1ekm.png

Template:STRUCTURE 1ekm

CRYSTAL STRUCTURE AT 2.5 A RESOLUTION OF ZINC-SUBSTITUTED COPPER AMINE OXIDASE OF HANSENULA POLYMORPHA EXPRESSED IN ESCHERICHIA COLI

Template:ABSTRACT PUBMED 10933787

About this Structure

1EKM is a Single protein structure of sequence from Pichia angusta. Full crystallographic information is available from OCA.

Reference

Crystal structure at 2.5 A resolution of zinc-substituted copper amine oxidase of Hansenula polymorpha expressed in Escherichia coli., Chen Z, Schwartz B, Williams NK, Li R, Klinman JP, Mathews FS, Biochemistry. 2000 Aug 15;39(32):9709-17. PMID:10933787

Page seeded by OCA on Tue Jul 1 00:54:02 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ekm"
Personal tools