6c54

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Current revision (14:26, 13 March 2024) (edit) (undo)
 
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<SX load='6c54' size='340' side='right' viewer='molstar' caption='[[6c54]], [[Resolution|resolution]] 5.80&Aring;' scene=''>
<SX load='6c54' size='340' side='right' viewer='molstar' caption='[[6c54]], [[Resolution|resolution]] 5.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6c54]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Zebov Zebov]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C54 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6C54 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6c54]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Zaire_ebolavirus Zaire ebolavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C54 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6C54 FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=186538 ZEBOV])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 5.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6c54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c54 OCA], [https://pdbe.org/6c54 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6c54 RCSB], [https://www.ebi.ac.uk/pdbsum/6c54 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6c54 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6c54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c54 OCA], [https://pdbe.org/6c54 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6c54 RCSB], [https://www.ebi.ac.uk/pdbsum/6c54 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6c54 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/NCAP_EBOZM NCAP_EBOZM] Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. During replication, encapsidation by NP is coupled to RNA synthesis and all replicative products are resistant to nucleases.
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Ebola virus nucleoprotein (eNP) assembles into higher-ordered structures that form the viral nucleocapsid (NC) and serve as the scaffold for viral RNA synthesis. However, molecular insights into the NC assembly process are lacking. Using a hybrid approach, we characterized the NC-like assembly of eNP, identified novel regulatory elements, and described how these elements impact function. We generated a three-dimensional structure of the eNP NC-like assembly at 5.8 A using electron cryo-microscopy and identified a new regulatory role for eNP helices alpha22-alpha23. Biochemical, biophysical, and mutational analyses revealed that inter-eNP contacts within alpha22-alpha23 are critical for viral NC assembly and regulate viral RNA synthesis. These observations suggest that the N terminus and alpha22-alpha23 of eNP function as context-dependent regulatory modules (CDRMs). Our current study provides a framework for a structural mechanism for NC-like assembly and a new therapeutic target.
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Electron Cryo-microscopy Structure of Ebola Virus Nucleoprotein Reveals a Mechanism for Nucleocapsid-like Assembly.,Su Z, Wu C, Shi L, Luthra P, Pintilie GD, Johnson B, Porter JR, Ge P, Chen M, Liu G, Frederick TE, Binning JM, Bowman GR, Zhou ZH, Basler CF, Gross ML, Leung DW, Chiu W, Amarasinghe GK Cell. 2018 Feb 22;172(5):966-978.e12. doi: 10.1016/j.cell.2018.02.009. PMID:29474922<ref>PMID:29474922</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6c54" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
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== References ==
 
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<references/>
 
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__TOC__
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[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Zebov]]
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[[Category: Zaire ebolavirus]]
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[[Category: Amarasinghe, G K]]
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[[Category: Amarasinghe GK]]
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[[Category: Chiu, W]]
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[[Category: Chiu W]]
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[[Category: Leung, D W]]
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[[Category: Leung DW]]
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[[Category: Pintilie, G D]]
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[[Category: Pintilie GD]]
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[[Category: Su, Z]]
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[[Category: Su Z]]
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[[Category: Wu, C]]
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[[Category: Wu C]]
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[[Category: Ebola]]
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[[Category: Helical reconstruction]]
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[[Category: Nucleocapsid]]
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[[Category: Nucleoprotein]]
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[[Category: Rna binding protein]]
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Current revision

Ebola nucleoprotein nucleocapsid-like assembly and the asymmetric unit

6c54, resolution 5.80Å

Proteopedia Page Contributors and Editors (what is this?)

OCA

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