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| - | [[Image:1elp.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1elp| PDB=1elp | SCENE= }} | | {{STRUCTURE_1elp| PDB=1elp | SCENE= }} |
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| - | '''GAMMA-D CRYSTALLIN STRUCTURE AT 1.95 A RESOLUTION'''
| + | ===GAMMA-D CRYSTALLIN STRUCTURE AT 1.95 A RESOLUTION=== |
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| - | ==Overview==
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| - | The crystal structure of bovine lens gammaIIIb-crystallin at 2.5 A resolution previously reported was interpreted using a consensus sequence derived from related vertebrate sequences on the assumption that gammaIIIb-crystallin derived from the gammaC-crystallin gene. It has recently been shown that gammaIIIb is a product of the bovine gammaD gene. The structure of gammaIIIb has now been refined with the bovine gammaD sequence using new 1.95 A resolution synchrotron data. The crystallographic R factor was 20.4% for all 33 104 reflection data between 8.0 and 1.95 A measured at 277(1) K. The electron density fully supported the assignment of the gammaD sequence to gammaIIIb. The crystal belongs to space group P2(1)2(1)2(1) with two molecules of molecular mass 20 749 Da in the asymmetric unit in which 219 water molecules were located. The two-domain four-Greek-key motif highly symmetrical protein is very similar in structure to gammaB-crystallin (81% sequence identity). There is a single amino-acid deletion in gammaD in the linker region connecting the two domains. The intermolecular oganization in the crystal lattice is quite different from gammaB as a result of key mutations involving surface residues Leu51, Ile103 and His155. These point mutations will contribute to the intermolecular behaviour of the gamma-crystallins in the eye lens, where they are major components of the densely packed, high refractive index regions of the lens.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_15299634}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 15299634 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_15299634}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Wright, G.]] | | [[Category: Wright, G.]] |
| | [[Category: Eye lens protein]] | | [[Category: Eye lens protein]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:15:12 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:56:55 2008'' |
Revision as of 21:56, 30 June 2008
Template:STRUCTURE 1elp
GAMMA-D CRYSTALLIN STRUCTURE AT 1.95 A RESOLUTION
Template:ABSTRACT PUBMED 15299634
About this Structure
1ELP is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
Structure of bovine eye lens gammaD (gammaIIIb)-crystallin at 1.95 A., Chirgadze YN, Driessen HP, Wright G, Slingsby C, Hay RE, Lindley PF, Acta Crystallogr D Biol Crystallogr. 1996 Jul 1;52(Pt 4):712-21. PMID:15299634
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