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2on8

From Proteopedia

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Current revision

Gbeta1 stabilization by in vitro evolution and computational design

Structural highlights

2on8 is a 1 chain structure with sequence from Streptococcus sp. G148. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.35Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SPG2_STRSG

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Computational design and in vitro evolution are major strategies for stabilizing proteins. For the four critical positions 16, 18, 25, and 29 of the B domain of the streptococcal protein G (Gbeta1), they identified the same optimal residues at positions 16 and 25, but not at 18 and 29. Here we analyzed the energetic contributions of the residues from these two approaches by single and double mutant analyses and determined crystal structures for a variant from the calculation (I16/L18/E25/K29) and from the selection (I16/I18/E25/F29). The structural analysis explains the observed differences in stabilization. Residues 16, 18, and 29 line an invagination, which results from a packing defect between the helix and the beta-sheet of Gbeta1. In all stabilized variants, residues with larger side-chains occur at these positions and packing is improved. In the selected variant, packing is better optimized than in the computed variant. Such differences in side-chain packing strongly affect stability but are difficult to evaluate by computation.

Optimization of the gbeta1 domain by computational design and by in vitro evolution: structural and energetic basis of stabilization.,Wunderlich M, Max KE, Roske Y, Mueller U, Heinemann U, Schmid FX J Mol Biol. 2007 Oct 26;373(3):775-84. Epub 2007 Aug 19. PMID:17868696^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wunderlich M, Max KE, Roske Y, Mueller U, Heinemann U, Schmid FX. Optimization of the gbeta1 domain by computational design and by in vitro evolution: structural and energetic basis of stabilization. J Mol Biol. 2007 Oct 26;373(3):775-84. Epub 2007 Aug 19. PMID:17868696 doi:http://dx.doi.org/10.1016/j.jmb.2007.08.004

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2on8"

Categories: Large Structures | Streptococcus sp. G148 | Heinemann U | Max KEA

@@ Line 3: / Line 3: @@
 <StructureSection load='2on8' size='340' side='right'caption='[[2on8]], [[Resolution|resolution]] 1.35&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2on8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_g148 Streptococcus g148]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ON8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ON8 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2on8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_sp._G148 Streptococcus sp. G148]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ON8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ON8 FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1pga|1pga]], [[1pgb|1pgb]], [[1gb4|1gb4]], [[1fcc|1fcc]], [[3gb1|3gb1]], [[1p7e|1p7e]]</div></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.35&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">spg ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1324 Streptococcus G148])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2on8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2on8 OCA], [https://pdbe.org/2on8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2on8 RCSB], [https://www.ebi.ac.uk/pdbsum/2on8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2on8 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/SPG2_STRSG SPG2_STRSG]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 35: / Line 36: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Streptococcus g148]]
+[[Category: Streptococcus sp. G148]]
-[[Category: Heinemann, U]]
+[[Category: Heinemann U]]
-[[Category: Max, K E.A]]
+[[Category: Max KEA]]
-[[Category: Alpha helix]]
-[[Category: Beta sheet]]
-[[Category: Improved hydrophobic packing of core residue]]
-[[Category: Protein binding]]

2on8

From Proteopedia

Current revision

Gbeta1 stabilization by in vitro evolution and computational design

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

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Proteopedia Page Contributors and Editors (what is this?)

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