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1epo

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[[Image:1epo.jpg|left|200px]]
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{{STRUCTURE_1epo| PDB=1epo | SCENE= }}
{{STRUCTURE_1epo| PDB=1epo | SCENE= }}
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'''DIRECT OBSERVATION BY X-RAY ANALYSIS OF THE TETRAHEDRAL "INTERMEDIATE" OF ASPARTIC PROTEINASES'''
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===DIRECT OBSERVATION BY X-RAY ANALYSIS OF THE TETRAHEDRAL "INTERMEDIATE" OF ASPARTIC PROTEINASES===
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==Overview==
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We report the X-ray analysis at 2.0 A resolution for crystals of the aspartic proteinase endothiapepsin (EC 3.4.23.6) complexed with a potent difluorostatone-containing tripeptide renin inhibitor (CP-81,282). The scissile bond surrogate, an electrophilic ketone, is hydrated in the complex. The pro-(R) (statine-like) hydroxyl of the tetrahedral carbonyl hydrate is hydrogen-bonded to both active-site aspartates 32 and 215 in the position occupied by a water in the native enzyme. The second hydroxyl oxygen of the hydrate is hydrogen-bonded only to the outer oxygen of Asp 32. These experimental data provide a basis for a model of the tetrahedral intermediate in aspartic proteinase-mediated cleavage of the amide bond. This indicates a mechanism in which Asp 32 is the proton donor and Asp 215 carboxylate polarizes a bound water for nucleophilic attack. The mechanism involves a carboxylate (Asp 32) that is stabilized by extensive hydrogen bonding, rather than an oxyanion derivative of the peptide as in serine proteinase catalysis.
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(as it appears on PubMed at http://www.pubmed.gov), where 1304340 is the PubMed ID number.
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{{ABSTRACT_PUBMED_1304340}}
==About this Structure==
==About this Structure==
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[[Category: Cooper, J B.]]
[[Category: Cooper, J B.]]
[[Category: Veerapandian, B.]]
[[Category: Veerapandian, B.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:22:56 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 01:32:30 2008''

Revision as of 22:32, 30 June 2008

Image:1epo.png

Template:STRUCTURE 1epo

DIRECT OBSERVATION BY X-RAY ANALYSIS OF THE TETRAHEDRAL "INTERMEDIATE" OF ASPARTIC PROTEINASES

Template:ABSTRACT PUBMED 1304340

About this Structure

1EPO is a Single protein structure. Full crystallographic information is available from OCA.

Reference

Direct observation by X-ray analysis of the tetrahedral "intermediate" of aspartic proteinases., Veerapandian B, Cooper JB, Sali A, Blundell TL, Rosati RL, Dominy BW, Damon DB, Hoover DJ, Protein Sci. 1992 Mar;1(3):322-8. PMID:1304340

Page seeded by OCA on Tue Jul 1 01:32:30 2008

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