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| - | [[Image:1epy.gif|left|200px]] | + | {{Seed}} |
| | + | [[Image:1epy.png|left|200px]] |
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| | {{STRUCTURE_1epy| PDB=1epy | SCENE= }} | | {{STRUCTURE_1epy| PDB=1epy | SCENE= }} |
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| - | '''T4 LYSOZYME MUTANT, T21H/C54T/C97A/Q141H/T142H'''
| + | ===T4 LYSOZYME MUTANT, T21H/C54T/C97A/Q141H/T142H=== |
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| - | ==Overview==
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| - | It is not easy to find candidate sites within a given protein where the geometry of the polypeptide chain matches that of metal-binding sites in known protein structures. By choosing a location in T4 lysozyme that is inherently flexible, it was possible to engineer a two-histidine site that binds different divalent cations. Crystallographic analysis shows that the geometry of binding of zinc is distorted tetrahedral while that of cobalt and nickel is octahedral. Insofar as spectroscopic data can be measured, they indicate that similar modes of coordination are retained in solution. The two substitutions, Thr21 --> His and Thr142 --> His, lie, respectively, on the surface of the N- and C-terminal domains on opposite sides of the active site cleft. The design takes advantage of hinge-bending motion which allows the binding site to adapt to the most favorable ligand geometry for the metal. Introduction of the two histidines increases the melting temperature of the protein by 2.0 degrees C at pH 7.4. Metal binding further increases the melting temperature, but only by a small amount (up to 1.5 degrees C). A third substitution, Gln141 --> His, which could act as a third ligand in principle, does not do so, demonstrating the difficulty in mimicking naturally occurring metal-binding sites.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_10835104}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 10835104 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_10835104}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Zhang, X J.]] | | [[Category: Zhang, X J.]] |
| | [[Category: Metal binding]] | | [[Category: Metal binding]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:23:22 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 01:36:28 2008'' |
Revision as of 22:36, 30 June 2008
Template:STRUCTURE 1epy
T4 LYSOZYME MUTANT, T21H/C54T/C97A/Q141H/T142H
Template:ABSTRACT PUBMED 10835104
About this Structure
1EPY is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.
Reference
Use of a non-rigid region in T4 lysozyme to design an adaptable metal-binding site., Wray JW, Baase WA, Ostheimer GJ, Zhang XJ, Matthews BW, Protein Eng. 2000 May;13(5):313-21. PMID:10835104
Page seeded by OCA on Tue Jul 1 01:36:28 2008
