1es8

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[[Image:1es8.jpg|left|200px]]
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[[Image:1es8.png|left|200px]]
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{{STRUCTURE_1es8| PDB=1es8 | SCENE= }}
{{STRUCTURE_1es8| PDB=1es8 | SCENE= }}
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'''CRYSTAL STRUCTURE OF FREE BGLII'''
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===CRYSTAL STRUCTURE OF FREE BGLII===
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==Overview==
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Restriction endonuclease BglII completely encircles its target DNA, making contacts to both the major and minor grooves. To allow the DNA to enter and leave the binding cleft, the enzyme dimer has to rearrange. To understand how this occurs, we have solved the structure of the free enzyme at 2.3 A resolution, as a complement to our earlier work on the BglII-DNA complex. Unexpectedly, the enzyme opens by a dramatic 'scissor-like' motion, accompanied by a complete rearrangement of the alpha-helices at the dimer interface. Moreover, within each monomer, a set of residues--a 'lever'--lowers or raises to alternately sequester or expose the active site residues. Such an extreme difference in free versus complexed structures has not been reported for other restriction endonucleases. This elegant mechanism for capturing DNA may extend to other enzymes that encircle DNA.
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The line below this paragraph, {{ABSTRACT_PUBMED_11175900}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11175900 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11175900}}
==About this Structure==
==About this Structure==
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[[Category: Restriction enzyme]]
[[Category: Restriction enzyme]]
[[Category: Uncomplexed]]
[[Category: Uncomplexed]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:27:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 01:47:55 2008''

Revision as of 22:48, 30 June 2008

Image:1es8.png

Template:STRUCTURE 1es8

CRYSTAL STRUCTURE OF FREE BGLII

Template:ABSTRACT PUBMED 11175900

About this Structure

1ES8 is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Structure of free BglII reveals an unprecedented scissor-like motion for opening an endonuclease., Lukacs CM, Kucera R, Schildkraut I, Aggarwal AK, Nat Struct Biol. 2001 Feb;8(2):126-30. PMID:11175900

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