This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1es8

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1es8.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1es8.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1es8| PDB=1es8 | SCENE= }}
{{STRUCTURE_1es8| PDB=1es8 | SCENE= }}
-
'''CRYSTAL STRUCTURE OF FREE BGLII'''
+
===CRYSTAL STRUCTURE OF FREE BGLII===
-
==Overview==
+
<!--
-
Restriction endonuclease BglII completely encircles its target DNA, making contacts to both the major and minor grooves. To allow the DNA to enter and leave the binding cleft, the enzyme dimer has to rearrange. To understand how this occurs, we have solved the structure of the free enzyme at 2.3 A resolution, as a complement to our earlier work on the BglII-DNA complex. Unexpectedly, the enzyme opens by a dramatic 'scissor-like' motion, accompanied by a complete rearrangement of the alpha-helices at the dimer interface. Moreover, within each monomer, a set of residues--a 'lever'--lowers or raises to alternately sequester or expose the active site residues. Such an extreme difference in free versus complexed structures has not been reported for other restriction endonucleases. This elegant mechanism for capturing DNA may extend to other enzymes that encircle DNA.
+
The line below this paragraph, {{ABSTRACT_PUBMED_11175900}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 11175900 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_11175900}}
==About this Structure==
==About this Structure==
Line 27: Line 31:
[[Category: Restriction enzyme]]
[[Category: Restriction enzyme]]
[[Category: Uncomplexed]]
[[Category: Uncomplexed]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:27:40 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 01:47:55 2008''

Revision as of 22:48, 30 June 2008

Image:1es8.png

Template:STRUCTURE 1es8

CRYSTAL STRUCTURE OF FREE BGLII

Template:ABSTRACT PUBMED 11175900

About this Structure

1ES8 is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Structure of free BglII reveals an unprecedented scissor-like motion for opening an endonuclease., Lukacs CM, Kucera R, Schildkraut I, Aggarwal AK, Nat Struct Biol. 2001 Feb;8(2):126-30. PMID:11175900

Page seeded by OCA on Tue Jul 1 01:47:55 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1es8"
Personal tools