1esb

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[[Image:1esb.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1esb| PDB=1esb | SCENE= }}
{{STRUCTURE_1esb| PDB=1esb | SCENE= }}
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'''DIRECT STRUCTURE OBSERVATION OF AN ACYL-ENZYME INTERMEDIATE IN THE HYDROLYSIS OF AN ESTER SUBSTRATE BY ELASTASE'''
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===DIRECT STRUCTURE OBSERVATION OF AN ACYL-ENZYME INTERMEDIATE IN THE HYDROLYSIS OF AN ESTER SUBSTRATE BY ELASTASE===
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==Overview==
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The method of X-ray crystallographic cryoenzymology has been used to determine the crystal structure of a kinetically significant species on the reaction pathway of a crystalline enzyme. The structure of a specific acyl-enzyme intermediate in the elastase-catalyzed hydrolysis of the N-carbobenzoxy-L-alanine p-nitrophenyl ester has been determined and refined against X-ray diffraction data at 2.3-A resolution. The difference Fourier electron density map clearly shows electron density for the trapped acyl-enzyme. The acyl-enzyme was formed at -26 degrees C and was stabilized at -55 degrees C during data collection, taking advantage of the glass transition in protein dynamics that occurs at around -50 degrees C.
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(as it appears on PubMed at http://www.pubmed.gov), where 8049229 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8049229}}
==About this Structure==
==About this Structure==
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[[Category: Rasmussen, B.]]
[[Category: Rasmussen, B.]]
[[Category: Ringe, D.]]
[[Category: Ringe, D.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 01:48:16 2008''

Revision as of 22:48, 30 June 2008

Image:1esb.png

Template:STRUCTURE 1esb

DIRECT STRUCTURE OBSERVATION OF AN ACYL-ENZYME INTERMEDIATE IN THE HYDROLYSIS OF AN ESTER SUBSTRATE BY ELASTASE

Template:ABSTRACT PUBMED 8049229

About this Structure

1ESB is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.

Reference

Direct structural observation of an acyl-enzyme intermediate in the hydrolysis of an ester substrate by elastase., Ding X, Rasmussen BF, Petsko GA, Ringe D, Biochemistry. 1994 Aug 9;33(31):9285-93. PMID:8049229

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