7a7e
From Proteopedia
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==Structure of a delta-N mutant - E232start - of PA1120 (TpbB or YfiN) from Pseudomonas aeruginosa (PAO1) comprising only the GGDEF domain== | ==Structure of a delta-N mutant - E232start - of PA1120 (TpbB or YfiN) from Pseudomonas aeruginosa (PAO1) comprising only the GGDEF domain== | ||
| - | <StructureSection load='7a7e' size='340' side='right'caption='[[7a7e]]' scene=''> | + | <StructureSection load='7a7e' size='340' side='right'caption='[[7a7e]], [[Resolution|resolution]] 2.80Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A7E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A7E FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7a7e]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A7E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A7E FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a7e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a7e OCA], [https://pdbe.org/7a7e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a7e RCSB], [https://www.ebi.ac.uk/pdbsum/7a7e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a7e ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a7e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a7e OCA], [https://pdbe.org/7a7e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a7e RCSB], [https://www.ebi.ac.uk/pdbsum/7a7e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a7e ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q9I4L5_PSEAE Q9I4L5_PSEAE] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | GGDEF-containing proteins respond to different environmental cues to finely modulate cyclic diguanylate (c-di-GMP) levels in time and space, making the allosteric control a distinctive trait of the corresponding proteins. The diguanylate cyclase mechanism is emblematic of this control: two GGDEF domains, each binding one GTP molecule, must dimerize to enter catalysis and yield c-di-GMP. The need for dimerization makes the GGDEF domain an ideal conformational switch in multidomain proteins. A re-evaluation of the kinetic profile of previously characterized GGDEF domains indicated that they are also able to convert GTP to GMP: this unexpected reactivity occurs when conformational issues hamper the cyclase activity. These results create new questions regarding the characterization and engineering of these proteins for in solution or structural studies. | ||
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| + | Studying GGDEF Domain in the Act: Minimize Conformational Frustration to Prevent Artefacts.,Mantoni F, Scribani Rossi C, Paiardini A, Di Matteo A, Cappellacci L, Petrelli R, Ricciutelli M, Paone A, Cutruzzola F, Giardina G, Rinaldo S Life (Basel). 2021 Jan 6;11(1). pii: life11010031. doi: 10.3390/life11010031. PMID:33418960<ref>PMID:33418960</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7a7e" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Diguanylate cyclase|Diguanylate cyclase]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Pseudomonas aeruginosa PAO1]] | ||
[[Category: Brunotti P]] | [[Category: Brunotti P]] | ||
[[Category: Giardina G]] | [[Category: Giardina G]] | ||
[[Category: Mantoni F]] | [[Category: Mantoni F]] | ||
[[Category: Rinaldo S]] | [[Category: Rinaldo S]] | ||
Current revision
Structure of a delta-N mutant - E232start - of PA1120 (TpbB or YfiN) from Pseudomonas aeruginosa (PAO1) comprising only the GGDEF domain
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