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2rqp

From Proteopedia

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The Solution Structure of Heterochromatin Protein 1-Binding Protein 74 Histone H1 like domain

Structural highlights

2rqp is a 1 chain structure with sequence from Homo sapiens. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

HP1B3_HUMAN Component of heterochromatin, may be involved in chromatin structure and function (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

In higher eukaryotic cells, DNA molecules are present as chromatin fibers, complexes of DNA with various types of proteins; chromatin fibers are highly condensed in metaphase chromosomes during mitosis. Although the formation of the metaphase chromosome structure is essential for the equal segregation of replicated chromosomal DNA into the daughter cells, the mechanism involved in the organization of metaphase chromosomes is poorly understood. To identify proteins involved in the formation and/or maintenance of metaphase chromosomes, we examined proteins that dissociated from isolated human metaphase chromosomes by 0.4 m NaCl treatment; this treatment led to significant chromosome decondensation, but the structure retained the core histones. One of the proteins identified, HP1-BP74 (heterochromatin protein 1-binding protein 74), composed of 553 amino acid residues, was further characterized. HP1-BP74 middle region (BP74Md), composed of 178 amino acid residues (Lys(97)-Lys(274)), formed a chromatosome-like structure with reconstituted mononucleosomes and protected the linker DNA from micrococcal nuclease digestion by approximately 25 bp. The solution structure determined by NMR revealed that the globular domain (Met(153)-Thr(237)) located within BP74Md possesses a structure similar to that of the globular domain of linker histones, which underlies its nucleosome binding properties. Moreover, we confirmed that BP74Md and full-length HP1-BP74 directly binds to HP1 (heterochromatin protein 1) and identified the exact sites responsible for this interaction. Thus, we discovered that HP1-BP74 directly binds to HP1, and its middle region associates with linker DNA at the entry/exit site of nucleosomal DNA in vitro.

The middle region of an HP1-binding protein, HP1-BP74, associates with linker DNA at the entry/exit site of nucleosomal DNA.,Hayashihara K, Uchiyama S, Shimamoto S, Kobayashi S, Tomschik M, Wakamatsu H, No D, Sugahara H, Hori N, Noda M, Ohkubo T, Zlatanova J, Matsunaga S, Fukui K J Biol Chem. 2010 Feb 26;285(9):6498-507. Epub 2009 Dec 30. PMID:20042602^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hayashihara K, Uchiyama S, Shimamoto S, Kobayashi S, Tomschik M, Wakamatsu H, No D, Sugahara H, Hori N, Noda M, Ohkubo T, Zlatanova J, Matsunaga S, Fukui K. The middle region of an HP1-binding protein, HP1-BP74, associates with linker DNA at the entry/exit site of nucleosomal DNA. J Biol Chem. 2010 Feb 26;285(9):6498-507. Epub 2009 Dec 30. PMID:20042602 doi:10.1074/jbc.M109.092833

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2rqp"

Categories: Homo sapiens | Large Structures | Ohkubo T | Shimamoto S | Sugahara H

@@ Line 1: / Line 1: @@
 ==The Solution Structure of Heterochromatin Protein 1-Binding Protein 74 Histone H1 like domain==
-<StructureSection load='2rqp' size='340' side='right'caption='[[2rqp]], [[NMR_Ensembles_of_Models | 12 NMR models]]' scene=''>
+<StructureSection load='2rqp' size='340' side='right'caption='[[2rqp]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2rqp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RQP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RQP FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2rqp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RQP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RQP FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HP1BP3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rqp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rqp OCA], [https://pdbe.org/2rqp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rqp RCSB], [https://www.ebi.ac.uk/pdbsum/2rqp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rqp ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/HP1B3_HUMAN HP1B3_HUMAN]] Component of heterochromatin, may be involved in chromatin structure and function (By similarity).
+[https://www.uniprot.org/uniprot/HP1B3_HUMAN HP1B3_HUMAN] Component of heterochromatin, may be involved in chromatin structure and function (By similarity).
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 32: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Ohkubo, T]]
+[[Category: Ohkubo T]]
-[[Category: Shimamoto, S]]
+[[Category: Shimamoto S]]
-[[Category: Sugahara, H]]
+[[Category: Sugahara H]]
-[[Category: Alternative splicing]]
-[[Category: Chromosomal protein]]
-[[Category: Dna-binding]]
-[[Category: Gene regulation]]
-[[Category: Heterochromatin protein 1]]
-[[Category: Heterochromatin protein 1 binding protein]]
-[[Category: Histone h1]]
-[[Category: Nucleus]]
-[[Category: Phosphoprotein]]

2rqp

From Proteopedia

Current revision

The Solution Structure of Heterochromatin Protein 1-Binding Protein 74 Histone H1 like domain

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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