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| | <StructureSection load='7ep8' size='340' side='right'caption='[[7ep8]], [[Resolution|resolution]] 1.95Å' scene=''> | | <StructureSection load='7ep8' size='340' side='right'caption='[[7ep8]], [[Resolution|resolution]] 1.95Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[7ep8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Neucr Neucr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EP8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7ep8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Neurospora_crassa_OR74A Neurospora crassa OR74A]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EP8 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pcn, NCU09239 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=367110 NEUCR])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95Å</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ep8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ep8 OCA], [https://pdbe.org/7ep8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ep8 RCSB], [https://www.ebi.ac.uk/pdbsum/7ep8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ep8 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ep8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ep8 OCA], [https://pdbe.org/7ep8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ep8 RCSB], [https://www.ebi.ac.uk/pdbsum/7ep8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ep8 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/Q7SF71_NEUCR Q7SF71_NEUCR]] This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.[RuleBase:RU000641]
| + | [https://www.uniprot.org/uniprot/Q7SF71_NEUCR Q7SF71_NEUCR] This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.[RuleBase:RU000641] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 7ep8" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 7ep8" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Proliferating cell nuclear antigen 3D structures|Proliferating cell nuclear antigen 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Neucr]] | + | [[Category: Neurospora crassa OR74A]] |
| - | [[Category: Sundaram, R]] | + | [[Category: Sundaram R]] |
| - | [[Category: Vasudevan, D]] | + | [[Category: Vasudevan D]] |
| - | [[Category: Dna binding protein]]
| + | |
| - | [[Category: Dna repair]]
| + | |
| - | [[Category: Dna replication]]
| + | |
| - | [[Category: Dna sliding clamp]]
| + | |
| Structural highlights
Function
Q7SF71_NEUCR This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.[RuleBase:RU000641]
Publication Abstract from PubMed
Eukaryotic proliferating cell nuclear antigen (PCNA) plays an essential role in orchestrating assembly of the replisome complex, stimulating processive DNA synthesis, and recruiting other regulatory proteins during the DNA damage response. PCNA and its binding partner network are relatively conserved in eukaryotes, and PCNA exhibits extraordinary structural similarity across species. However, despite this structural similarity, the PCNA of a given species is rarely functional in heterologous systems. In this report, we determined the X-ray crystal structure of Neurospora crassa PCNA and compared its structure-function relationship with other available PCNA studies to understand this cross-species incompatibility. We found two regions, the interdomain connecting loop (IDCL) and J loop structures, vary significantly among PCNAs. In particular, the J loop deviates in NcPCNA from that in Saccharomyces cerevisiae PCNA by 7 A. Differences in the IDCL structures result in varied binding affinities of PCNAs for the subunit Pol32 of DNA polymerase delta and for T2AA, a small molecule inhibitor of human PCNA. To validate that these structural differences are accountable for functional incompatibility in S. cerevisiae, we generated NcPCNA mutants mimicking IDCL and J loop structures of ScPCNA. Our genetic analyses suggested that NcPCNA mutants are fully functional in S. cerevisiae. The susceptibility of the strains harboring ScPCNA mimics of NcPCNA to various genotoxic agents was similar to that in yeast cells expressing ScPCNA. Taken together, we conclude that in addition to the overall architecture of PCNA, structures of the IDCL and J loop of PCNA are critical determinants of interspecies functional compatibility.
Interdomain connecting loop and J loop structures determine cross-species compatibility of PCNA.,Kumari P, Sundaram R, Manohar K, Vasudevan D, Acharya N J Biol Chem. 2021 Jun 24:100911. doi: 10.1016/j.jbc.2021.100911. PMID:34175309[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kumari P, Sundaram R, Manohar K, Vasudevan D, Acharya N. Interdomain connecting loop and J loop structures determine cross-species compatibility of PCNA. J Biol Chem. 2021 Jun 24:100911. doi: 10.1016/j.jbc.2021.100911. PMID:34175309 doi:http://dx.doi.org/10.1016/j.jbc.2021.100911
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