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| | ==SEQUENTIAL 1H,13C AND 15N NMR ASSIGNMENTS AND SOLUTION CONFORMATION OF APOKEDARCIDIN== | | ==SEQUENTIAL 1H,13C AND 15N NMR ASSIGNMENTS AND SOLUTION CONFORMATION OF APOKEDARCIDIN== |
| - | <StructureSection load='1akp' size='340' side='right'caption='[[1akp]], [[NMR_Ensembles_of_Models | 15 NMR models]]' scene=''> | + | <StructureSection load='1akp' size='340' side='right'caption='[[1akp]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1akp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Actsl Actsl]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AKP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AKP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1akp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Actinomycete_ATCC_53650 Actinomycete ATCC 53650]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AKP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AKP FirstGlance]. <br> |
| | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1akp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1akp OCA], [https://pdbe.org/1akp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1akp RCSB], [https://www.ebi.ac.uk/pdbsum/1akp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1akp ProSAT]</span></td></tr> | | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1akp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1akp OCA], [https://pdbe.org/1akp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1akp RCSB], [https://www.ebi.ac.uk/pdbsum/1akp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1akp ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/KEDA_ACTSL KEDA_ACTSL]] Binds non-covalently to an enediyne chromophore which is the cytotoxic and mutagenic component of the antibiotic. The chromophore cleaves duplex DNA site-specifically in a single-stranded manner. The apoprotein cleaves proteins selectively, in particular highly basic histones, with H1 proteins being cleaved the more readily.
| + | [https://www.uniprot.org/uniprot/KEDA_ACTSL KEDA_ACTSL] Binds non-covalently to an enediyne chromophore which is the cytotoxic and mutagenic component of the antibiotic. The chromophore cleaves duplex DNA site-specifically in a single-stranded manner. The apoprotein cleaves proteins selectively, in particular highly basic histones, with H1 proteins being cleaved the more readily. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Actsl]] | + | [[Category: Actinomycete ATCC 53650]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bruccoleri, R E]] | + | [[Category: Bruccoleri RE]] |
| - | [[Category: Colson, K L]] | + | [[Category: Colson KL]] |
| - | [[Category: Constantine, K L]] | + | [[Category: Constantine KL]] |
| - | [[Category: Friedrichs, M S]] | + | [[Category: Farmer II BT]] |
| - | [[Category: II, B T.Farmer]] | + | [[Category: Friedrichs MS]] |
| - | [[Category: Lam, K S]] | + | [[Category: Lam KS]] |
| - | [[Category: Langley, D R]] | + | [[Category: Langley DR]] |
| - | [[Category: Leet, J E]] | + | [[Category: Leet JE]] |
| - | [[Category: Metzler, W J]] | + | [[Category: Metzler WJ]] |
| - | [[Category: Mueller, L]] | + | [[Category: Mueller L]] |
| - | [[Category: Schroeder, D R]] | + | [[Category: Schroeder DR]] |
| - | [[Category: Tuttle, J]] | + | [[Category: Tuttle J]] |
| - | [[Category: Wittekind, M]] | + | [[Category: Wittekind M]] |
| - | [[Category: Zein, N]] | + | [[Category: Zein N]] |
| - | [[Category: Antibiotic chromoprotein]]
| + | |
| Structural highlights
Function
KEDA_ACTSL Binds non-covalently to an enediyne chromophore which is the cytotoxic and mutagenic component of the antibiotic. The chromophore cleaves duplex DNA site-specifically in a single-stranded manner. The apoprotein cleaves proteins selectively, in particular highly basic histones, with H1 proteins being cleaved the more readily.
Publication Abstract from PubMed
Kedarcidin is a recently discovered antitumor antibiotic chromoprotein. The solution conformation of the kedarcidin apoprotein (114 residues) has been characterized by heteronuclear multidimensional NMR spectroscopy. Sequence-specific backbone atom resonance assignments were obtained for a uniformly 13C/15N-enriched sample of apokedarcidin via a semiautomated analysis of 3D HNCACB, 3D CBCA-(CO)NH, 4D HNCAHA, 4D HN(CO)CAHA, 3D HBHA(CO)NH, and 3D HNHA(Gly) spectra. Side-chain assignments were subsequently obtained by analysis of (primarily) 3D HCCH-TOCSY and HCCH-COSY spectra. A qualitative analysis of the secondary structure is presented on the basis of 3J alpha NH coupling constants, deviations of 13C alpha and 13C beta chemical shifts from random coil values, and NOEs observed in 3D 15N- and 13C-edited NOESY-HSQC spectra. This analysis revealed a four-stranded antiparallel beta-sheet, a three-stranded antiparallel beta-sheet, and two two-standed antiparallel beta-sheets. The assignments of cross-peaks in the 3D NOESY spectra were assisted by reference to a preliminary model of apokedarcidin built using the program CONGEN starting from the X-ray structure of the homologous protein aponeocarzinostatin. An ensemble of 15 apokedarcidin solution structures has been generated by variable target function minimization (DIANA program) and refined by simulated annealing (X-PLOR program). The average backbone atom root-mean-square difference between the individual structures and the mean coordinates is 0.68 +/- 0.08 A. The overall fold of apokedarcidin is well-defined; it is composed of an immunoglobulin-like seven-stranded antiparallel beta-barrel and a subdomain containing two antiparallel beta-ribbons. Highly similar tertiary structures have been previously reported for the related proteins neocarzinostatin, macromomycin, and actinoxanthin. Important structural features are revealed, including the dimensions of the chromophore-binding pocket and the locations of side chains that are likely to be involved in chromophore stabilization.
Sequential 1H, 13C, and 15N NMR assignments and solution conformation of apokedarcidin.,Constantine KL, Colson KL, Wittekind M, Friedrichs MS, Zein N, Tuttle J, Langley DR, Leet JE, Schroeder DR, Lam KS, et al. Biochemistry. 1994 Sep 27;33(38):11438-52. PMID:7918358[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Constantine KL, Colson KL, Wittekind M, Friedrichs MS, Zein N, Tuttle J, Langley DR, Leet JE, Schroeder DR, Lam KS, et al.. Sequential 1H, 13C, and 15N NMR assignments and solution conformation of apokedarcidin. Biochemistry. 1994 Sep 27;33(38):11438-52. PMID:7918358
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